Kusunose Masahiko, Qiu Bing, Cui Tailin, Hamada Atsuhide, Yoshioka Saburo, Ono Masahide, Miyamura Mitsuhiko, Kyotani Shojiro, Nishioka Yutaka
Department of Pharmacy, Kochi Medical School Hospital, Kochi, Japan.
Biol Pharm Bull. 2002 Nov;25(11):1417-21. doi: 10.1248/bpb.25.1417.
Sho-saiko-to extract, a Chinese herbal medicine, is widely used for treatment of chronic hepatitis in Japan. However, it is not clear what conditions Sho-saiko-to extract improves hepatic inflammation and fibrosis. We therefore induced various stages of liver injury in model rats and administered Sho-saiko-to extract. We then evaluated the liver inflammation and liver fibrosis-improving effects of Sho-saiko-to extract. The liver injury model rats were produced by administration of various doses of dimethylnitrosamine (DMN) and Sho-saiko-to extract was administered to these rats. Then the liver inflammation and fibrosis-improving effects of Sho-saiko-to extract were evaluated according to L-asparate aminotransferase (AST), L-alanine aminotransferase (ALT), liver retinoid levels, levels of hydroxyproline, Transforming Growth Factor-beta (TGF-beta), and the liver fibrosis area. These indicators depended on the total doses of DMN. The ability of Sho-saiko-to extract to improve liver inflammation and fibrosis was limited to the following levels of the respective parameters: AST levels (234-264 U/l), ALT levels (208-232 U/l), TGF-beta levels (1102-1265 pg/g liver tissue), hydroxyproline levels (633-719 nmol/g liver tissue), and liver fibrosis area (9.7-10.6 times for normal rat). These findings suggested that Sho-saiko-to extract is effective in the treatment of liver inflammation and fibrosis up to a certain degree of severity, but it produces no improvement in more severe cases.
小柴胡汤提取物是一种中药，在日本被广泛用于治疗慢性肝炎。然而，尚不清楚小柴胡汤提取物在何种条件下可改善肝脏炎症和纤维化。因此，我们在模型大鼠中诱导了不同阶段的肝损伤，并给予小柴胡汤提取物。然后，我们评估了小柴胡汤提取物对肝脏炎症和肝纤维化的改善作用。通过给予不同剂量的二甲基亚硝胺（DMN）制备肝损伤模型大鼠，并给这些大鼠服用小柴胡汤提取物。然后根据L-天门冬氨酸氨基转移酶（AST）、L-丙氨酸氨基转移酶（ALT）、肝脏类视黄醇水平、羟脯氨酸水平、转化生长因子-β（TGF-β）以及肝纤维化面积来评估小柴胡汤提取物对肝脏炎症和纤维化的改善作用。这些指标取决于DMN的总剂量。小柴胡汤提取物改善肝脏炎症和纤维化的能力仅限于以下各自参数水平：AST水平（234 - 264 U/l）、ALT水平（208 - 232 U/l）、TGF-β水平（1102 - 1265 pg/g肝组织）、羟脯氨酸水平（633 - 719 nmol/g肝组织）以及肝纤维化面积（为正常大鼠的9.7 - 10.6倍）。这些发现表明，小柴胡汤提取物在治疗一定严重程度的肝脏炎症和纤维化方面是有效的，但在更严重的病例中并无改善作用。
