Abe Fumiko, Yamauchi Tatsuo, Nagao Tsuneatsu, Kinjo Junei, Okabe Hikaru, Higo Hiroo, Akahane Hiroshige
Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
Biol Pharm Bull. 2002 Nov;25(11):1485-7. doi: 10.1248/bpb.25.1485.
The MeOH extract of the leaves of rosemary (Rosmarinus officinalis L.) completely inhibited the motility of cultured epimastigotes of Trypanosoma cruzi at the concentration of 2 mg/ml after 2 h of incubation. Activity-guided fractionation of the MeOH extract has resulted in the isolation of three triterpene acids, betulinic, oleanolic and ursolic acids. Ursolic acid stopped the movement of all T. cruzi epimastigotes at the minimum concentration (MC(100)) of 40 micro g/ml (88 micro M) after 48 h of incubation. Oleanolic acid was less active (MC(100): 250 micro g/ml, 550 micro M) and betulinic acid was practically inactive.
迷迭香叶的甲醇提取物在浓度为2mg/ml、孵育2小时后，完全抑制了克氏锥虫培养型上鞭毛体的运动能力。对该甲醇提取物进行活性导向分级分离，得到了三种三萜酸，即桦木酸、齐墩果酸和熊果酸。熊果酸在孵育48小时后，以40μg/ml（88μM）的最低浓度（MC(100)）使所有克氏锥虫上鞭毛体停止运动。齐墩果酸的活性较低（MC(100)：250μg/ml，550μM），而桦木酸实际上无活性。
