Kelishadi Roya, Nadery Gholam Ali, Asgary Sedigheh
Isfahan Cardiovascular Research Center, Isfahan University of Medical Sciences, Iran.
Indian J Pediatr. 2002 Sep;69(9):755-9. doi: 10.1007/BF02723684.
Considering the importance of primary prevention of Cardiovascular Disease (CVD) from childhood, especially in children with high family risk for premature atherosclerosis, and also the importance of oxidized LDL in the process of atherosclerosis, the main metabolites of ox-LDL i.e. Malondialdehyde (MDA) and Conjugated diene (CDE) have been measured in children of high risk families and compared with a control group.
Children and adolescents (6-18 years) of parents with premature myocardial infarction (MI < or = 55 y in men and < or = 65 y in women), were selected as the case group. The control group included neighbors of the case group matched for age and socioeconomic status. All samples have been selected by simple random sampling. Both the case and control groups were divided in two subgroups: those with a total cholesterol and/or LDL-C > or = 95th centile and those with normal lipid levels. Each subgroup consisted of 32 subjects, so 128 subjects were studied (64 in the case and 64 in the control group). MDA and CDE were measured by spectrophotometry using molar absorbivity. Data were analyzed by SPSSv10/Win software using ANOVA, Bonferroni, Scheffe-Duncan, Tukey-HSD, and the Student's t-test.
The mean MDA value in the case and control groups was significantly different (1.84 +/- 0.43 vs. 1.67 +/- 0.41 micromol/L, p=0.03), but this difference was not significant regarding the mean CDE level (0.50 +/- 0.05 vs. 0.47 +/- 0.04 micromol/L, p>0.05). The mean MDA level in the case group with hyperlipidemia was significantly higher than that in the case group without hyperlipidemia (1.985 +/- 0.516 vs. 1.690 +/- 0.366, micromol/L, P=0.02) and also higher than control group with or without hyperlipidemia (1.985 +/- 0.516 vs. 1.720 +/- 0.389, 1.615 +/- 0.429 micromol/L respectively, P<0.05). The mean CDE level in the case group with hyperlipidemia was significantly higher than the case group without hyperlipidemia (0.542 +/- 0.034 vs. 0.494 +/- 0.049 micromol/L, P=0.04) and higher than the control group with or without hyperlipidemia (0.542 +/- 0.034 vs. 0.464 +/- 0.051, 0.484 +/- 0.048 micromol/L respectively, p<0.05). In case boys with hyperlipidemia, the mean MDA (2.03 +/- 0.2 micromol/L) and the mean of CDE (0.56 +/- 0.04 micromol/L) was significantly higher than other subgroups (P<0.05).
Considering the increased susceptibility of LDL to oxidation in children with high family risk for premature CVD, special attention should be paid to consumption of foods and seasoning containing antioxidants from childhood especially in high risk families.
鉴于从儿童期开始进行心血管疾病（CVD）一级预防的重要性，特别是在有早发动脉粥样硬化高家族风险的儿童中，以及氧化型低密度脂蛋白（ox-LDL）在动脉粥样硬化过程中的重要性，本研究对高风险家庭儿童的ox-LDL主要代谢产物即丙二醛（MDA）和共轭二烯（CDE）进行了测定，并与对照组进行比较。
选取父母患有早发心肌梗死（男性心肌梗死年龄≤55岁，女性≤65岁）的儿童和青少年（6 - 18岁）作为病例组。对照组包括年龄和社会经济地位相匹配的病例组邻居。所有样本均通过简单随机抽样选取。病例组和对照组均分为两个亚组：总胆固醇和/或低密度脂蛋白胆固醇（LDL-C）≥第95百分位数者以及血脂水平正常者。每个亚组由32名受试者组成，共研究128名受试者（病例组64名，对照组64名）。采用分光光度法利用摩尔吸光率测定MDA和CDE。使用SPSSv10/Win软件通过方差分析、Bonferroni检验、Scheffe-Duncan检验、Tukey-HSD检验和学生t检验对数据进行分析。
病例组和对照组的平均MDA值存在显著差异（1.84±0.43 vs. 1.67±0.41μmol/L，p = 0.03），但平均CDE水平差异不显著（0.50±0.05 vs. 0.47±0.04μmol/L，p>0.05）。高脂血症病例组的平均MDA水平显著高于无高脂血症的病例组（1.985±0.516 vs. 1.690±0.366，μmol/L，P = 0.02），也高于有或无高脂血症的对照组（分别为1.985±0.516 vs. 1.720±0.389，1.615±0.429μmol/L，P<0.05）。高脂血症病例组的平均CDE水平显著高于无高脂血症的病例组（0.542±0.034 vs. 0.494±0.049μmol/L，P = 0.04），也高于有或无高脂血症的对照组（分别为0.542±0.034 vs. 0.464±0.051，0.484±0.048μmol/L，p<0.05）。在患有高脂血症的男性病例中，平均MDA（2.03±0.2μmol/L）和平均CDE（0.56±0.04μmol/L）显著高于其他亚组（P<0.05）。
鉴于有早发CVD高家族风险的儿童中LDL氧化易感性增加，应从儿童期开始特别关注含抗氧化剂食物和调味料的摄入，尤其是在高风险家庭中。
