Mohanaradhakrishnan V, Muthiah P L, Hadhanyi A
Arzneimittelforschung. 1975 May;25(5):726-35.
A knowledge of the influence of several factors on the stability and properties of collagen and preparations thereof precedes their industrial application. The results of such a study are reported in this paper. The mechanical properties of skin samples vary significantly with location and direction of cutting. The properties of isolated fibres from these locations, however, did not differ significantly, in a single animal. Collagen fibres from tendons and skins of several mammalian and other species exhibited under identical conditions of testing differences in their physical properties. The influence of factors like composition, shrinkage temperature, non-collagenous components, fibril width and age, on the properties is discussed individually. A slight increase in strength was observed in deaminated and deguanidinated collagen fibres while all the other treatments studied, viz. modification of side chains, crosslinking, tanning and irradiation with gamma-rays decreased the strength. An inverse correlation was observed between shrinkage temperature and strength of treated collagen fibres. The strength was also found to be influenced by the morphological structure of collagen. Irradiation with gamma-rays caused a reduction of not only the strength and shrinkage temperature of collagen but also caused severe destruction of the fibrillar structure at the X-ray and electronmicroscopic levels. The degradation was progressive with dosage. Non-hydrolytic scission of the polypeptide chains seemed to be responsible for the changes observed, which were also species specific.
在胶原蛋白及其制剂进行工业应用之前，需要了解多种因素对其稳定性和性质的影响。本文报道了该项研究的结果。皮肤样本的力学性能会因切割位置和方向的不同而有显著差异。然而，在同一只动物身上，从这些位置分离出的纤维的性能并无显著差异。来自几种哺乳动物及其他物种的肌腱和皮肤中的胶原纤维，在相同测试条件下，其物理性能存在差异。本文分别讨论了组成、收缩温度、非胶原成分、原纤维宽度和年龄等因素对性能的影响。脱氨和脱胍的胶原纤维强度略有增加，而所研究的所有其他处理方式，即侧链修饰、交联、鞣制和γ射线辐照，均会降低强度。经处理的胶原纤维的收缩温度与强度之间呈负相关。还发现强度受胶原形态结构的影响。γ射线辐照不仅会降低胶原蛋白的强度和收缩温度，还会在X射线和电子显微镜水平上导致纤维结构的严重破坏。这种降解随剂量增加而加剧。多肽链的非水解断裂似乎是造成上述变化的原因，而且这些变化还具有物种特异性。
