Dumont Larry J, Beddard Rachel, Whitley Pamela, Johnson Adrienne, McNeil Deanna, Sawyer Sherrie, Elfath M Dean
Gambro BCT, Inc., 10811 West CollinsAvenue, Lakewood, CO 80215, USA.
Transfusion. 2002 Oct;42(10):1333-9. doi: 10.1046/j.1537-2995.2002.00222.x.
This study evaluates the recovery and survival of high-concentration platelets (HCPs) compared to standard apheresis platelets (APCs) in a double-label autologous human system.
Nine HCP units paired with APC units were stored, labeled with either 51Cr and 111In, and returned, and recovery and survival were determined. Standard in vitro platelet biochemical and functional parameters were monitored over the storage period and evaluated in a secondary analysis.
Three each HCP units containing more than 2.2 x 10(11), 1.5 x 10(11) to 2.1 x 10(11), and 0.8 x 10(11) to 1.1 x 10(11) platelets in 59.4 +/- 2.5 mL were stored for 1, 2, or 5 days, respectively, and simultaneously with matched APC units (3.8 x 10(11) platelets, 282 mL). Recoveries were 72.3 +/- 8.6, 60.8 +/- 14.6, and 52.5 +/- 6.7 percent for HCPs, respectively; and 59.4 +/- 6.4 percent for APCs (p=0.37). HCP survivals were 202.0 +/- 14.9, 204.9 +/- 10.2, and 162.6 +/- 17.0 hours; APC survivals were 155.4 +/- 20.3 hours (p=0.001). Secondary analysis with P-selectin added as a predictor in the model resulted in significant difference in recoveries for Day 1 HCPs versus Day 5 APCs (p=0.024) with no difference shown for HCPs on Days 2 or 5 versus APCs. No significant difference was found in survival (p=0.16).
HCPs may be stored 24 hours for high yield, 48 hours for intermediate yield, and up to 5 days for yields less than 1.6 x 10(11) platelets per bag with equivalent to superior recovery and survival of platelets in the autologous transfusion model compared to APCs.
本研究在双标记自体人类系统中，评估了高浓度血小板（HCP）与标准单采血小板（APC）相比的回收率和存活率。
将9个HCP单位与APC单位配对储存，用51Cr和111In进行标记后回输，并测定回收率和存活率。在储存期间监测标准的体外血小板生化和功能参数，并在二次分析中进行评估。
三个HCP单位分别含有超过2.2×10¹¹、1.5×10¹¹至2.1×10¹¹以及0.8×10¹¹至1.1×10¹¹个血小板，体积为59.4±2.5 mL，分别储存1天、2天或5天，同时与匹配的APC单位（3.8×10¹¹个血小板，282 mL）储存。HCP的回收率分别为72.3±8.6%、60.8±14.6%和52.5±6.7%；APC的回收率为59.4±6.4%（p = 0.37）。HCP的存活时间分别为202.0±14.9小时、204.9±10.2小时和162.6±17.0小时；APC的存活时间为155.4±20.3小时（p = 0.001）。在模型中加入P-选择素作为预测因子的二次分析显示，第1天的HCP与第5天的APC在回收率上有显著差异（p = 0.024），而第2天或第5天的HCP与APC之间无差异。在存活率方面未发现显著差异（p = 0.16）。
在自体输血模型中，与APC相比，HCP每袋血小板产量高于1.6×10¹¹时可储存24小时以获得高产量，产量为中等时可储存48小时，产量低于1.6×10¹¹时最多可储存5天，且血小板回收率和存活率相当或更优。
