Dumont Larry J, AuBuchon James P, Whitley Pamela, Herschel Louise H, Johnson Adrienne, McNeil Deanna, Sawyer Sherrie, Roger Jill C
Gambro BCT, Inc, Lakewood, Colorado 80215, USA.
Transfusion. 2002 Jul;42(7):847-54. doi: 10.1046/j.1537-2995.2002.00147.x.
Bacterial screening may effectively reduce the morbidity and mortality risk associated with extended storage of platelets. Platelet viability then becomes the primary determinant of acceptable storage time. This study evaluates the effectiveness of platelets stored in plasma for 7 days.
WBC-reduced, single-donor platelets (n = 24) were collected and stored by standard methods at two sites. Standard in vitro platelet biochemical and functional parameters were monitored over the storage period. On Days 5 and 7 of storage, platelets were alternately labeled with 51Cr and (111)In and returned to the subject, and recovery and survival were determined.
Component pH(22 degrees C) was maintained in the range 6.2 to 7.61 through 7 days and did not detrimentally affect either in vitro or in vivo outcomes. In vitro platelet characteristics were adequately maintained over 7 days. Day 5 platelets had better recovery (63.0 +/- 4.36 vs. 53.9 +/- 4.36%, p < 0.0001) and survival (161 +/- 8.1 vs. 133 +/- 8.1 hr, p = 0.006) than Day 7 platelets adjusting for radioisotope, center, and donor effects.
Although declines in recovery and survival were noted, these are less than used previously to gain licensure of 7-day storage and are unlikely to be clinically significant. Extension of storage to 7 days could be implemented with bacterial screening methods to select out contaminated components without a significant effect on the platelet efficacy compared to 5-day components.
细菌筛查可有效降低与血小板长期储存相关的发病和死亡风险。血小板活力随即成为可接受储存时间的主要决定因素。本研究评估了在血浆中储存7天的血小板的有效性。
收集24份白细胞去除的单供体血小板，在两个地点采用标准方法进行储存。在储存期间监测标准的体外血小板生化和功能参数。在储存的第5天和第7天，血小板分别用51Cr和（111）In交替标记后回输给受试者，并测定回收率和存活率。
在7天内，成分pH（22℃）维持在6.2至7.61范围内，对体外和体内结果均无不利影响。体外血小板特性在7天内得到充分维持。校正放射性同位素、中心和供体效应后，第5天的血小板比第7天的血小板具有更好的回收率（63.0±4.36%对53.9±4.36%，p<0.0001）和存活率（161±8.1小时对133±8.1小时，p=0.006）。
虽然观察到回收率和存活率有所下降，但这些下降幅度小于先前用于获得7天储存许可的幅度，且不太可能具有临床意义。与5天的成分相比，采用细菌筛查方法将储存期延长至7天，在挑选出受污染成分的同时，对血小板疗效无显著影响。
