Lima T M, Kanunfre C C, Pompéia C, Verlengia R, Curi R
Thais Martins de Lima, Departamento de Fisiologia e Biofísica, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1524, 05508-900, Butantã, São Paulo, SP, Brazil.
Toxicol In Vitro. 2002 Dec;16(6):741-7. doi: 10.1016/s0887-2333(02)00095-4.
The fatty acids have an important role in the control of leukocyte metabolism and function. Higher concentrations of certain fatty acids, particularly polyunsaturated fatty acids (PUFAs) and volatile fatty acids, can cause cell death via apoptosis or, when concentrations are greater, necrosis. In this study, we determined the highest concentrations of various fatty acids that are non-toxic to two human leukemic cell lines, Jurkat (T-lymphocyte) and Raji (B-lymphocyte). Toxicity was evaluated by either loss of membrane integrity and/or DNA fragmentation using flow cytometric analysis. There were no remarkable differences for the toxicity of the fatty acids between B and T cell lines. The cytotoxicity of the fatty acids was related to the carbon chain length and number of double bonds: docosahexaenoic acid=eicosapentaenoic acid=arachidonic acid=gamma-linolenic acid=stearic acid=palmitic acid > linoleic acid=palmitoleic acid > vacenic acid=lauric acid > oleic acid > elaidic acid > capric acid > butyric acid > caprylic acid=caproic acid=propionic acid. The proportion of cells undergoing apoptosis or necrosis, induced by the fatty acids tested, remains to be investigated.
脂肪酸在白细胞代谢和功能的调控中发挥着重要作用。某些脂肪酸，特别是多不饱和脂肪酸（PUFAs）和挥发性脂肪酸的浓度升高时，可通过凋亡导致细胞死亡，浓度更高时则会导致坏死。在本研究中，我们测定了对两种人类白血病细胞系Jurkat（T淋巴细胞）和Raji（B淋巴细胞）无毒的各种脂肪酸的最高浓度。使用流式细胞术分析通过膜完整性丧失和/或DNA片段化来评估毒性。B细胞系和T细胞系之间脂肪酸的毒性没有显著差异。脂肪酸的细胞毒性与碳链长度和双键数量有关：二十二碳六烯酸=二十碳五烯酸=花生四烯酸=γ-亚麻酸=硬脂酸=棕榈酸＞亚油酸=棕榈油酸＞反油酸=月桂酸＞油酸＞反式油酸＞癸酸＞丁酸＞辛酸=己酸=丙酸。所测试的脂肪酸诱导的细胞凋亡或坏死比例仍有待研究。
