Asakura Atsushi, Rudnicki Michael A
Ottawa Health Research Institute, Molecular Medicine Program, Ottawa, Ontario, Canada.
Exp Hematol. 2002 Nov;30(11):1339-45. doi: 10.1016/s0301-472x(02)00954-2.
Pluripotent hematopoietic stem cells and muscle-derived hematopoietic potential cells isolated by Hoechst 33342 dye-mediated fluorescein-activated cell sorting (FACS) as side population (SP) cells, give rise to hematopoietic cells as well as skeletal muscle cells following intravenous transplantation. However, besides bone marrow and skeletal muscle, it has remained unclear whether other adult tissues also contain SP cells that are enriched for cells that exhibit hematopoietic potential.
To test whether adult tissues contain SP cells with hematopoietic potential, Hoechst-FACS analysis and hematopoietic colony formation assays were performed with cells isolated from a variety of adult tissues, skeletal muscle, heart, brain, spleen, liver, kidney, lung, and small intestine and compared with peripheral blood and bone marrow cells.
In addition to hematopoietic tissues, cell preparations from nonhematopoietic tissues, such as skeletal muscle, kidney, lung, and small intestine, displayed markedly higher hematopoietic colony formation activity compared to peripheral blood cells. Moreover, the hematopoietic progenitors in these adult tissues expressed the hematopoietic cell marker CD45. Hoechst-FACS analysis demonstrated that all adult tissues examined contained SP cells. In addition, these SP fractions were enriched for cells that efficiently formed hematopoietic colonies in vitro.
These results indicate that hematopoietic progenitors are present in significant numbers in all adult tissues examined.
通过Hoechst 33342染料介导的荧光激活细胞分选（FACS）分离出的多能造血干细胞和肌肉来源的具有造血潜能的细胞，即边缘群（SP）细胞，在静脉注射移植后可产生造血细胞以及骨骼肌细胞。然而，除了骨髓和骨骼肌外，其他成年组织是否也含有富含具有造血潜能细胞的SP细胞仍不清楚。
为了检测成年组织是否含有具有造血潜能的SP细胞，对从多种成年组织（骨骼肌、心脏、脑、脾脏、肝脏、肾脏、肺和小肠）分离出的细胞进行了Hoechst-FACS分析和造血集落形成试验，并与外周血和骨髓细胞进行比较。
除造血组织外，来自非造血组织（如骨骼肌、肾脏、肺和小肠）的细胞制剂与外周血细胞相比，显示出明显更高的造血集落形成活性。此外，这些成年组织中的造血祖细胞表达造血细胞标志物CD45。Hoechst-FACS分析表明，所有检测的成年组织都含有SP细胞。此外，这些SP组分富含在体外能有效形成造血集落的细胞。
这些结果表明，在所有检测的成年组织中都存在大量的造血祖细胞。
