• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

体外分化 CD45-/CD31+ 肺侧群细胞为内皮和平滑肌细胞。

Differentiation of CD45‑/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro.

机构信息

Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China.

Department of Hematology, Jining First People's Hospital, Jining, Shandong 272000, P.R. China.

出版信息

Int J Mol Med. 2019 Mar;43(3):1128-1138. doi: 10.3892/ijmm.2019.4053. Epub 2019 Jan 8.

DOI:10.3892/ijmm.2019.4053
PMID:30628669
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6365051/
Abstract

Side population (SP) cells are a small subpopulation of cells found in many mammalian tissues and organs, identified by their capacity to efflux Hoechst 33342 dye. They are enriched for stem/progenitor cell activity. SP cells isolated from the adult mouse lung can be separated into a CD45+ subset (bone marrow‑derived) and a CD45‑ subset that can be subdivided into CD31‑ and CD31+ subpopulations. CD45‑/CD31‑ lung SP (LSP) cells are known to be mesenchymal stem cells. However, CD45‑/CD31+ LSP cells are not fully characterized. In the present study, it was found that CD45‑/CD31+ LSP cells were able to form colonies. Based on the expression of vascular endothelial growth factor receptor 2 (VEGFR2), these cells were separated into VEGFR2‑ and VEGFR2+ cells. The CD45‑/CD31+/VEGFR2‑ LSP cells expressed genes characteristic of smooth muscle and endothelial progenitors, and were able to differentiate into smooth muscle and endothelial cells in vitro. The CD45‑/CD31+/VEGFR2+ LSP cells expressed genes characteristic of endothelial progenitors and gave rise to endothelial cells, although not smooth muscle, in vitro. The data demonstrate that CD45‑/CD31+/VEGFR2‑ LSP cells differentiated into CD45‑/CD31+/VEGFR2+ LSP cells and then endothelial cells, indicating that CD45‑/CD31+/VEGFR2+ LSP cells are likely to be derived from CD45‑/CD31+/VEGFR2‑ LSP cells. Taken together, the results suggest that CD45‑/CD31+ LSP cells can be separated into CD45‑/CD31+/VEGFR2‑ LSP cells, which may be progenitors of endothelial and smooth muscle, whereas CD45‑/CD31+/VEGFR2+ LSP cells may serve as late commitment endothelial progenitors in the adult mouse lung.

摘要

侧群 (SP) 细胞是存在于许多哺乳动物组织和器官中的一小部分细胞，其特征是能够排出 Hoechst 33342 染料。它们富含干细胞/祖细胞活性。从成年小鼠肺部分离的 SP 细胞可分为 CD45+亚群（骨髓来源）和 CD45-亚群，后者可进一步分为 CD31-和 CD31+亚群。已知 CD45-/CD31-肺 SP（LSP）细胞是间充质干细胞。然而，CD45-/CD31+LSP 细胞尚未得到充分表征。在本研究中，发现 CD45-/CD31+LSP 细胞能够形成集落。基于血管内皮生长因子受体 2 (VEGFR2) 的表达，将这些细胞分为 VEGFR2-和 VEGFR2+细胞。CD45-/CD31+/VEGFR2-LSP 细胞表达平滑肌和内皮祖细胞特征基因，并能够在体外分化为平滑肌和内皮细胞。CD45-/CD31+/VEGFR2+LSP 细胞表达内皮祖细胞特征基因，并在体外产生内皮细胞，但不是平滑肌细胞。数据表明，CD45-/CD31+/VEGFR2-LSP 细胞分化为 CD45-/CD31+/VEGFR2+LSP 细胞，然后分化为内皮细胞，表明 CD45-/CD31+/VEGFR2+LSP 细胞可能来源于 CD45-/CD31+/VEGFR2-LSP 细胞。综上所述，结果表明 CD45-/CD31+LSP 细胞可分为 CD45-/CD31+/VEGFR2-LSP 细胞，其可能是内皮和平滑肌的祖细胞，而 CD45-/CD31+/VEGFR2+LSP 细胞可能是成年小鼠肺中晚期内皮祖细胞的定向分化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c975/6365051/a794954bb107/IJMM-43-03-1128-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c975/6365051/08d2da6b5645/IJMM-43-03-1128-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c975/6365051/4ae8b2b1c0af/IJMM-43-03-1128-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c975/6365051/87363b3d9da3/IJMM-43-03-1128-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c975/6365051/aa8b1997f9b5/IJMM-43-03-1128-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c975/6365051/569905370a67/IJMM-43-03-1128-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c975/6365051/2c2b1053faff/IJMM-43-03-1128-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c975/6365051/a794954bb107/IJMM-43-03-1128-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c975/6365051/08d2da6b5645/IJMM-43-03-1128-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c975/6365051/4ae8b2b1c0af/IJMM-43-03-1128-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c975/6365051/87363b3d9da3/IJMM-43-03-1128-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c975/6365051/aa8b1997f9b5/IJMM-43-03-1128-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c975/6365051/569905370a67/IJMM-43-03-1128-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c975/6365051/2c2b1053faff/IJMM-43-03-1128-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c975/6365051/a794954bb107/IJMM-43-03-1128-g06.jpg

相似文献

1
Differentiation of CD45‑/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro.体外分化 CD45-/CD31+ 肺侧群细胞为内皮和平滑肌细胞。
Int J Mol Med. 2019 Mar;43(3):1128-1138. doi: 10.3892/ijmm.2019.4053. Epub 2019 Jan 8.
2
[Differentiation of Cultivated CD45/CD31 Mouse Lung Side Population Cells into Vascular Smooth Muscle Cells ].[培养的CD45/CD31小鼠肺侧群细胞向血管平滑肌细胞的分化]
Sichuan Da Xue Xue Bao Yi Xue Ban. 2017 May;48(3):363-367.
3
Embryonic lung side population cells are hematopoietic and vascular precursors.胚胎肺侧群细胞是造血和血管前体细胞。
Am J Respir Cell Mol Biol. 2005 Jul;33(1):32-40. doi: 10.1165/rcmb.2005-0024OC. Epub 2005 Mar 31.
4
In vitro and in vivo proliferation, differentiation and migration of cardiac endothelial progenitor cells (SCA1+/CD31+ side-population cells).心脏内皮祖细胞（SCA1+/CD31+侧群细胞）的体外和体内增殖、分化和迁移。
J Thromb Haemost. 2011 Aug;9(8):1628-37. doi: 10.1111/j.1538-7836.2011.04375.x.
5
Endothelial and smooth muscle cells derived from human cardiac explants demonstrate angiogenic potential and suitable for design of cell-containing vascular grafts.源自人心脏外植体的内皮细胞和平滑肌细胞具有血管生成潜力，适用于含细胞血管移植物的设计。
J Transl Med. 2017 Mar 3;15(1):54. doi: 10.1186/s12967-017-1156-1.
6
Functional Definition of Progenitors Versus Mature Endothelial Cells Reveals Key SoxF-Dependent Differentiation Process.祖细胞与成熟内皮细胞的功能定义揭示了关键的 SoxF 依赖性分化过程。
Circulation. 2017 Feb 21;135(8):786-805. doi: 10.1161/CIRCULATIONAHA.116.024754. Epub 2016 Nov 29.
7
The mouse endometrium contains epithelial, endothelial and leucocyte populations expressing the stem cell marker telomerase reverse transcriptase.小鼠子宫内膜包含表达干细胞标志物端粒酶逆转录酶的上皮细胞、内皮细胞和白细胞群体。
Mol Hum Reprod. 2016 Apr;22(4):272-84. doi: 10.1093/molehr/gav076. Epub 2016 Jan 5.
8
The differentiation of mesenchymal stem cells to vascular cells regulated by the HMGB1/RAGE axis: its application in cell therapy for transplant arteriosclerosis.HMGB1/RAGE 轴调控间充质干细胞向血管细胞的分化：在移植性动脉硬化细胞治疗中的应用。
Stem Cell Res Ther. 2018 Apr 3;9(1):85. doi: 10.1186/s13287-018-0827-z.
9
Proliferation, differentiation and migration of SCA1/CD31 cardiac side population cells in vitro and in vivo.SCA1/CD31心脏侧群细胞在体内外的增殖、分化和迁移
Int J Cardiol. 2017 Jan 15;227:378-386. doi: 10.1016/j.ijcard.2016.11.047. Epub 2016 Nov 9.
10
Endothelial progenitor cells derived from CD34+ cells form cooperative vascular networks.源自CD34+细胞的内皮祖细胞形成协作性血管网络。
Cell Physiol Biochem. 2010;26(4-5):679-88. doi: 10.1159/000322335. Epub 2010 Oct 29.

引用本文的文献

1
Endothelial transdifferentiation of glioma stem cells: a literature review.胶质瘤干细胞的内皮转分化：文献综述
Acta Neuropathol Commun. 2025 Aug 21;13(1):181. doi: 10.1186/s40478-025-02031-x.
2
Exploring extramedullary hematopoiesis: unraveling the hematopoietic microenvironments.探索髓外造血：解析造血微环境
Front Hematol. 2024;3. doi: 10.3389/frhem.2024.1371823. Epub 2024 May 28.
3
Protrudin regulates FAK activation, endothelial cell migration and angiogenesis.Protrudin 调节 FAK 的激活、内皮细胞迁移和血管生成。

本文引用的文献

1
Proliferation, differentiation and migration of SCA1/CD31 cardiac side population cells in vitro and in vivo.SCA1/CD31心脏侧群细胞在体内外的增殖、分化和迁移
Int J Cardiol. 2017 Jan 15;227:378-386. doi: 10.1016/j.ijcard.2016.11.047. Epub 2016 Nov 9.
2
The Function of SDF-1-CXCR4 Axis in SP Cells-Mediated Protective Role for Renal Ischemia/Reperfusion Injury by SHH/GLI1-ABCG2 Pathway.SDF-1-CXCR4轴在SP细胞通过SHH/GLI1-ABCG2途径介导的肾缺血/再灌注损伤保护作用中的功能
Shock. 2017 Feb;47(2):251-259. doi: 10.1097/SHK.0000000000000694.
3
Targeting of the pulmonary capillary vascular niche promotes lung alveolar repair and ameliorates fibrosis.
Cell Mol Life Sci. 2022 Apr 4;79(4):220. doi: 10.1007/s00018-022-04251-z.
4
Generation of Spike-Extracellular Vesicles (S-EVs) as a Tool to Mimic SARS-CoV-2 Interaction with Host Cells. Spike-Extracellular Vesicles (S-EVs) 的产生可作为模拟 SARS-CoV-2 与宿主细胞相互作用的工具。
Cells. 2022 Jan 3;11(1):146. doi: 10.3390/cells11010146.
5
Characteristics and clinical significance of CD163+/CD206+M2 mono-macrophage in the bladder cancer microenvironment.膀胱癌微环境中CD163+/CD206+M2单巨噬细胞的特征及临床意义
Turk J Biol. 2021 Oct 18;45(5):624-632. doi: 10.3906/biy-2104-17. eCollection 2021.
6
Current concepts on endothelial stem cells definition, location, and markers.目前关于内皮祖细胞定义、位置和标志物的概念。
Stem Cells Transl Med. 2021 Nov;10 Suppl 2(Suppl 2):S54-S61. doi: 10.1002/sctm.21-0022.
7
An open-label phase 1 clinical trial of the allogeneic side population adipose-derived mesenchymal stem cells in SMA type 1 patients.1型脊髓性肌萎缩症患者异体侧群脂肪间充质干细胞的开放标签1期临床试验。
Neurol Sci. 2022 Jan;43(1):399-410. doi: 10.1007/s10072-021-05291-2. Epub 2021 May 25.
8
Programming to S1PR1 Endothelial Cells Promotes Restoration of Vascular Integrity.编程 S1PR1 内皮细胞促进血管完整性的恢复。
Circ Res. 2021 Jul 9;129(2):221-236. doi: 10.1161/CIRCRESAHA.120.318412. Epub 2021 Apr 30.
9
Loss of endothelial cell-specific autophagy-related protein 7 exacerbates doxorubicin-induced cardiotoxicity.内皮细胞特异性自噬相关蛋白7的缺失会加剧阿霉素诱导的心脏毒性。
Biochem Biophys Rep. 2021 Jan 30;25:100926. doi: 10.1016/j.bbrep.2021.100926. eCollection 2021 Mar.
靶向肺毛细血管血管生态位可促进肺泡修复并改善纤维化。
Nat Med. 2016 Feb;22(2):154-62. doi: 10.1038/nm.4035. Epub 2016 Jan 18.
4
c-kit(+) cells adopt vascular endothelial but not epithelial cell fates during lung maintenance and repair.c-kit(+) 细胞在肺维持和修复过程中采用血管内皮细胞而不是上皮细胞命运。
Nat Med. 2015 Aug;21(8):866-8. doi: 10.1038/nm.3888. Epub 2015 Jul 13.
5
Ventilator Strategies and Rescue Therapies for Management of Acute Respiratory Failure in the Emergency Department.急诊科急性呼吸衰竭管理中的通气策略与挽救治疗
Ann Emerg Med. 2015 Nov;66(5):529-41. doi: 10.1016/j.annemergmed.2015.04.030. Epub 2015 May 23.
6
Rifampicin-dependent antibodies target glycoprotein IIb/IIIa and cause clearance of human platelets in NOD/SCID mice.利福平依赖性抗体靶向糖蛋白IIb/IIIa并导致NOD/SCID小鼠体内人血小板清除。
Br J Haematol. 2016 Jan;172(1):137-40. doi: 10.1111/bjh.13470. Epub 2015 Apr 29.
7
Pericytes, mesenchymal stem cells and their contributions to tissue repair.周细胞、间充质干细胞及其在组织修复中的作用。
Pharmacol Ther. 2015 Jul;151:107-20. doi: 10.1016/j.pharmthera.2015.03.006. Epub 2015 Mar 28.
8
Perivascular Gli1+ progenitors are key contributors to injury-induced organ fibrosis.血管周围Gli1+祖细胞是损伤诱导器官纤维化的关键促成因素。
Cell Stem Cell. 2015 Jan 8;16(1):51-66. doi: 10.1016/j.stem.2014.11.004. Epub 2014 Nov 20.
9
Reviews and prospectives of signaling pathway analysis in idiopathic pulmonary fibrosis.特发性肺纤维化中信号通路分析的研究进展与展望。
Autoimmun Rev. 2014 Oct;13(10):1020-5. doi: 10.1016/j.autrev.2014.08.028. Epub 2014 Aug 23.
10
ABCG2pos lung mesenchymal stem cells are a novel pericyte subpopulation that contributes to fibrotic remodeling.ABCG2阳性肺间充质干细胞是一种新型周细胞亚群，有助于纤维化重塑。
Am J Physiol Cell Physiol. 2014 Oct 15;307(8):C684-98. doi: 10.1152/ajpcell.00114.2014.