Guillén Llera J L, López García M L, Martín Reinoso E, De Vivar González R
Escuela de Especialización, Profesional de Análisis Clínicos, Dpto Inmunología, Facultad de Farmacia, Universidad Complutense, Avda Complutense s/n, 28040, Madrid, Spain.
Vet Parasitol. 2002 Nov 11;109(3-4):185-90. doi: 10.1016/s0304-4017(02)00306-0.
A mixed indirect fluorescence antibody test (IFAT), based on cultured promastigotes Leishmania infantum and formol-inactivated suspension of cells infected with the bacteria Ehrlichia canis, was applied to make a differential diagnosis between canine ehrlichiosis and leishmaniosis. A titre greater than 80 was considered positive for antibodies to E. canis and suggestive of antibodies to L. infantum. Positive sera were titrated subsequently by serial dilutions to confirm antibodies positive to Leishmania and establishing the antibody titre of both pathogens. Fluorescence was absent with negative control sera and background staining was minimal. No serological cross-reactions between positive sera for L. infantum or E. canis were detected. Results obtained by mixed IFAT did not differ when the same serum IFAT standard was compared. The test showed equivalent sensitivity (100%). The specifities were 100% for L. infantum and 98.5% for E. canis. The equivalence in sensitivity was confirmed by calculating the correlation coefficient between IFAT standards and mixed IFAT (r>or=0.99 for both pathogens). The results of our investigations demonstrated that mixed IFAT is a specific means of establishing serological differential diagnosis of canine leishmaniosis and ehrlichiosis.
基于培养的婴儿利什曼原虫前鞭毛体和犬埃立克体感染细胞的甲醛灭活悬液，进行了混合间接荧光抗体试验（IFAT），以对犬埃立克体病和利什曼病进行鉴别诊断。犬埃立克体抗体滴度大于80被认为呈阳性，并提示存在婴儿利什曼原虫抗体。随后通过系列稀释对阳性血清进行滴定，以确认利什曼原虫抗体阳性，并确定两种病原体的抗体滴度。阴性对照血清无荧光，背景染色极少。未检测到婴儿利什曼原虫或犬埃立克体阳性血清之间的血清学交叉反应。当比较相同血清IFAT标准时，混合IFAT获得的结果没有差异。该试验显示出同等的敏感性（100%）。婴儿利什曼原虫的特异性为100%，犬埃立克体的特异性为98.5%。通过计算IFAT标准与混合IFAT之间的相关系数（两种病原体的r≥0.99），证实了敏感性的等效性。我们的研究结果表明，混合IFAT是建立犬利什曼病和埃立克体病血清学鉴别诊断的一种特异性方法。
