Krawczak Felipe da Silva, Reis Ilka Afonso, Silveira Julia Angélica da, Avelar Daniel Moreira, Marcelino Andreza Pain, Werneck Guilherme Loureiro, Labruna Marcelo Bahia, Paz Gustavo Fontes
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil.
Departamento de Estatística, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Rev Soc Bras Med Trop. 2015 Jan-Feb;48(1):64-8. doi: 10.1590/0037-8682-0291-2014. Epub 2015 Jan 1.
The present study was designed to assess the occurrence of co-infection or cross-reaction in the serological techniques used for detecting the anti-Leishmania spp., -Babesia canis vogeli and -Ehrlichia canis antibodies in urban dogs from an area endemic to these parasites.
The serum samples from dogs were tested for the Babesia canis vogeli strain Belo Horizonte antigen and Ehrlichia canis strain São Paulo by immunofluorescence antibody test (IFAT) and by anti-Leishmania immunoglobulin G (IgG) antibody detection to assess Leishmania infection. We used the following four commercial kits for canine visceral leishmaniasis: ELISA, IFAT, Dual Path Platform (DPP) (Bio Manguinhos(r)/FIOCRUZ/MS) and a rK39 RDT (Kalazar Detect Canine Rapid Test; Inbios).
Of 96 serum samples submitted to serological assays, 4 (4.2%) were positive for Leishmania as determined by ELISA; 12 (12.5%), by IFAT; 14 (14.6%) by rK39 RDT; and 20 (20.8%), by DPP. Antibodies against Ehrlichia and Babesia were detected in 23/96 (23.9%) and 30/96 (31.2%) samples, respectively. No significant association was identified between the results of tests for detecting Babesia or Ehrlichia and those for detecting Leishmania (p-value>0.05).
In the present study, we demonstrated co-infection with Ehrlichia or Babesia and Leishmania in dogs from Minas Gerais (Brazil); we also found that the serological tests that were used did not cross-react.
本研究旨在评估在用于检测来自这些寄生虫流行地区城市犬体内抗利什曼原虫属、犬巴贝斯虫沃氏亚种和犬埃立克体抗体的血清学技术中，共感染或交叉反应的发生情况。
通过免疫荧光抗体试验（IFAT）检测犬血清样本中的犬巴贝斯虫沃氏亚种贝洛奥里藏特株抗原和犬埃立克体圣保罗株，并通过检测抗利什曼原虫免疫球蛋白G（IgG）抗体来评估利什曼原虫感染情况。我们使用了以下四种用于犬内脏利什曼病的商业试剂盒：酶联免疫吸附测定（ELISA）、免疫荧光抗体试验（IFAT）、双路平台（DPP）（Bio Manguinhos(r)/FIOCRUZ/MS）和rK39快速诊断试验（黑热病检测犬用快速试验；Inbios）。
在接受血清学检测的96份血清样本中，ELISA检测出4份（4.2%）利什曼原虫阳性；IFAT检测出12份（12.5%）阳性；rK39快速诊断试验检测出14份（14.6%）阳性；DPP检测出20份（20.8%）阳性。分别在23/96（23.9%）和30/96（31.2%）的样本中检测到抗埃立克体和抗巴贝斯虫抗体。在检测巴贝斯虫或埃立克体的试验结果与检测利什曼原虫的试验结果之间未发现显著关联（p值>0.05）。
在本研究中，我们证明了巴西米纳斯吉拉斯州的犬存在埃立克体或巴贝斯虫与利什曼原虫的共感染；我们还发现所使用的血清学检测没有交叉反应。
