Oliveira Trícia Maria F de Sousa, Furuta Patrícia I, de Carvalho Débora, Machado Rosangela Z
Curso de Pós-Graduação em Medicina Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Campus de Jaboticabal, Jaboticabal, SP, Brazil.
Rev Bras Parasitol Vet. 2008 Jan-Mar;17(1):7-11. doi: 10.1590/s1984-29612008000100002.
To verify the presence of cross-reaction among leishmaniosis, ehrlichiosis and babesiosis in serological diagnostics used in human visceral leishmaniasis control programs, serum samples from leishmaniasis endemic and non-endemic areas were collected and tested by Indirect Fluorescent Antibody (IFAT) and Enzyme-linked immunosorbent assay (ELISA). All serum samples from endemic areas were positive for Leishmania sp., by ELISA and IFAT, 51% positive for Babesia canis and 43% for Ehrlichia canis by IFAT. None of the serum samples from non-endemic areas were positive for Leishmania sp., by IFAT, but 67% were positive for B. canis and 78% for E. canis using the same test. When tested by ELISA for Leishmania sp., four samples from non-endemic area were positive. These dogs were then located and no clinical signs, parasites or antibody was detected in new tests for a six month period. Only one of these 4 samples was positive for B. canis by IFAT and ELISA and three for E. canis by IFAT. The results of the work suggest a co-infection in the endemic area and no serological cross-reaction among these parasites by IFAT and ELISA.
为了验证在人类内脏利什曼病控制项目中使用的血清学诊断中利什曼病、埃立克体病和巴贝斯虫病之间是否存在交叉反应，收集了利什曼病流行区和非流行区的血清样本，并通过间接荧光抗体试验（IFAT）和酶联免疫吸附测定（ELISA）进行检测。流行区的所有血清样本通过ELISA和IFAT检测利什曼原虫均呈阳性，通过IFAT检测犬巴贝斯虫阳性率为51%，犬埃立克体阳性率为43%。非流行区的血清样本通过IFAT检测利什曼原虫均为阴性，但使用相同检测方法检测犬巴贝斯虫阳性率为67%，犬埃立克体阳性率为78%。通过ELISA检测利什曼原虫时，非流行区的4份样本呈阳性。随后找到了这些犬，在为期6个月的新检测中未发现临床症状、寄生虫或抗体。这4份样本中只有1份通过IFAT和ELISA检测犬巴贝斯虫呈阳性，3份通过IFAT检测犬埃立克体呈阳性。该研究结果表明流行区存在共同感染，并且通过IFAT和ELISA检测这些寄生虫之间不存在血清学交叉反应。
