Günter Elena A, Ovodov Yury S
Institute of Physiology, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, 50, Pervomaiskaya strasse, 167982, Syktyvkar, Russia.
Carbohydr Res. 2002 Oct 8;337(18):1641-5. doi: 10.1016/s0008-6215(02)00079-4.
Pectin termed silenan and acidic arabinogalactan were isolated as cell-wall polysaccharides of Silene vulgaris callus in the presence of various carbon sources as components of the media. The maximum yields, productivity per litre of medium and production per day of acidic arabinogalactan, were achieved using glucose or galactose as the carbon source. Sucrose was found to increase the production of the polysaccharides. Yields, productivity and rate of production of arabinogalactan per day were decreased in the presence of arabinose. Yields of silenan, productivity and rate of production per day were closely related irrespective of the sugar used as the carbon source in the media (sucrose, glucose or galactose) and yields of silenan from the callus growing on arabinose were comparable. A concentration of sucrose in the 20-50 g/L range enhanced the biosynthesis of silenan and at 50 g/L the silenan contained the linear backbone and the ramified regions of the macromolecule.
在不同碳源作为培养基成分存在的情况下，从蝇子草愈伤组织中分离出了名为硅烷聚糖的果胶和酸性阿拉伯半乳聚糖作为细胞壁多糖。以葡萄糖或半乳糖作为碳源时，酸性阿拉伯半乳聚糖的产量、每升培养基的生产率和每日产量达到最高。发现蔗糖可增加多糖的产量。在阿拉伯糖存在的情况下，阿拉伯半乳聚糖的产量、生产率和每日产率均降低。无论培养基中用作碳源的糖是蔗糖、葡萄糖还是半乳糖，硅烷聚糖的产量、生产率和每日产率都密切相关，并且在阿拉伯糖上生长的愈伤组织中硅烷聚糖的产量相当。20 - 50 g/L范围内的蔗糖浓度可增强硅烷聚糖的生物合成，在50 g/L时，硅烷聚糖包含大分子的线性主链和分支区域。
