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[普通麦瓶草细胞培养物的多糖]

[Polysaccharides of cell cultures of Silene vulgaris].

作者信息

Giunter E A, Ovodov Iu S

出版信息

Prikl Biokhim Mikrobiol. 2007 Jan-Feb;43(1):94-101.

PMID:17345866
Abstract

Callus and suspension cultures of campion (Silene vulgaris) produced pectin polysaccharides, similar in structure to the polysaccharides of intact plants. The major components of the pectins were D-galacturonic acid, galactose, arabinose, and rhamnose residues. The maximum content of pectins was found in callus. The monosaccharide composition of arabinogalactans isolated from cells and a culture medium of callus cultures were similar, with the ratio between arabinose and galactose of 1: (2.3-6.5) being retained. The arabinogalactans from the cells and culture medium of the suspension cultures also had a similar structure, and the arabinose to galactose ratio was 1: (1.5-1.8). In contrast to the callus cultures, the suspension cultures produced arabinogalactans with an increased content of arabinose residues and a decreased content of galactose residues. The greatest content of arabinogalactan was detected in the culture medium of the suspension cultures.

摘要

剪秋罗(蝇子草)的愈伤组织和悬浮培养物产生了果胶多糖,其结构与完整植株的多糖相似。果胶的主要成分是D-半乳糖醛酸、半乳糖、阿拉伯糖和鼠李糖残基。在愈伤组织中发现果胶含量最高。从愈伤组织培养物的细胞和培养基中分离出的阿拉伯半乳聚糖的单糖组成相似,阿拉伯糖与半乳糖的比例保持在1:(2.3 - 6.5)。悬浮培养物的细胞和培养基中的阿拉伯半乳聚糖也具有相似的结构,阿拉伯糖与半乳糖的比例为1:(1.5 - 1.8)。与愈伤组织培养物不同,悬浮培养物产生的阿拉伯半乳聚糖中阿拉伯糖残基含量增加,半乳糖残基含量减少。在悬浮培养物的培养基中检测到的阿拉伯半乳聚糖含量最高。

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