Günter E A, Ovodov Yu S
Institute of Physiology, Komi Science Center, The Urals Branch of the Russian Academy of Sciences, Syktyvkar 167982, Russia.
Biochemistry (Mosc). 2003 Aug;68(8):882-9. doi: 10.1023/a:1025751015684.
Sources of carbohydrate nutrition such as sucrose, glucose, and galactose, with the exception of arabinose, were shown to influence positively callus growth and polysaccharide (pectin silenan and acidic arabinogalactan) biosynthesis. Galactose was found to cause a stimulatory effect on yield and productivity of arabinogalactan. Low concentrations of sucrose failed to support the cell growth and polysaccharide biosynthesis. Increasing sucrose concentrations led to biomass accumulation but failed to enhance efficiency of the substrate utilization. The optimal medium for the campion cell culture growth was found to be one containing 30 g/liter of sucrose or a mixture of sucrose with glucose (in 15 g/liter). Increasing sucrose concentrations in the medium from 30 to 100 g/liter failed to significantly influence the polysaccharide yields while the polysaccharide productivity per liter of the medium grew due to promotion of culture productivity in biomass. Variations of the carbon sources in the nutrient media were shown to influence insignificantly the biochemical characteristics of arabinogalactan and silenan while an increase in the sucrose concentration to 50-100 g/liter led to a diminution of the galacturonic acid content in silenan and to changes in contents of the neutral monosaccharide residues in silenan and arabinogalactan.
除阿拉伯糖外，蔗糖、葡萄糖和半乳糖等碳水化合物营养来源被证明对愈伤组织生长和多糖（果胶硅烷醇和酸性阿拉伯半乳聚糖）生物合成有积极影响。发现半乳糖对阿拉伯半乳聚糖的产量和生产率有刺激作用。低浓度的蔗糖无法支持细胞生长和多糖生物合成。蔗糖浓度的增加导致生物量积累，但未能提高底物利用效率。发现用于剪秋罗属植物细胞培养生长的最佳培养基是含有30克/升蔗糖或蔗糖与葡萄糖混合物（15克/升）的培养基。培养基中蔗糖浓度从30克/升增加到100克/升对多糖产量没有显著影响，而每升培养基的多糖生产率因生物量中培养生产率的提高而增加。营养培养基中碳源的变化对阿拉伯半乳聚糖和硅烷醇生化特性的影响不显著，而蔗糖浓度增加到50 - 100克/升会导致硅烷醇中半乳糖醛酸含量减少以及硅烷醇和阿拉伯半乳聚糖中中性单糖残基含量的变化。
