采用富含5/B抗原复合物的纯化抗原，通过酶联免疫吸附测定法对包虫病特异性IgG、IgM和IgA进行手术前后检测。
Pre- and postsurgical detection of IgG, IgM, and IgA specific to hydatidosis by ELISA with purified antigen enriched with the 5/B antigen complex.
作者信息
Doiz Olga, Benito Rafael, Gil Joaquina, Rojas Almudena, Rubio M Carmen, Osuna Antonio
机构信息
Servicio de Microbiología, Hospital Clínico Universitario, Zaragoza, España.
出版信息
J Clin Lab Anal. 2002;16(6):295-8. doi: 10.1002/jcla.10056.
Abstract
An enzyme-linked immunoassay (ELISA) using purified 5/B Echinococcus enriched antigen was used to follow IgG, IgM, and IgA antibody levels pre- and posttreatment or surgical removal of hydatid cysts. The sensitivity was 97%, 37.5%, and 54.5%, respectively, and the specificity was 95.7%, 100%, and 98.9%, respectively. All isotypes could be detected 3 years after surgical removal of cysts in patients showing no remaining cyst evidence. This was especially true for IgG, which persisted in 85.2% of the patients. The data indicate that antigen purification improves specificity without affecting sensitivity, although this new antigen offers no advantages in the postsurgical monitoring of the patients.
摘要
采用纯化的多房棘球绦虫富集抗原的酶联免疫吸附测定（ELISA）来追踪治疗前及治疗后或手术切除包虫囊肿前后的IgG、IgM和IgA抗体水平。其敏感性分别为97%、37.5%和54.5%，特异性分别为95.7%、100%和98.9%。在无残留囊肿证据的患者中，囊肿手术切除3年后可检测到所有免疫球蛋白类型。IgG尤其如此，85.2%的患者体内IgG持续存在。数据表明，抗原纯化可提高特异性而不影响敏感性，尽管这种新抗原在患者术后监测中并无优势。
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