Ho Cheong-Yip, Wong Chun-Kwok, Ko Fanny Wai-San, Chan Christopher Hok-Sum, Ho Alice Sheng-Sheng, Hui David Shu-Cheong, Lam Christopher Wai-Kei
Departments of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong.
Chest. 2002 Nov;122(5):1751-8. doi: 10.1378/chest.122.5.1751.
s: The dysregulation of apoptosis and the expression of apoptosis-related molecules of allergen-reactive T lymphocytes have been suggested to play a key role in the development and maintenance of the inflammatory reactions in allergic asthma. Glucocorticoids are effective drugs for treating allergic inflammation. In this study, we investigated the effect of dexamethasone (DEX) on the apoptosis and B-cell lymphoma (Bcl)-2 expression of peripheral blood T lymphocytes as well as the soluble form of Fas (sFas) in allergic asthmatic patients.
Peripheral blood lymphocytes from 41 allergic asthmatic patients and 30 age-matched and sex-matched control subjects were treated with 0.1 and 1 micro M DEX. The percentages of apoptosis and expression of the Bcl-2 molecule in T lymphocytes were assessed by flow cytometry. The plasma concentration of sFas was measured using the enzyme-linked immunosorbent assay.
DEX (0.1 and 1 micro M) could induce the apoptosis of T lymphocytes from allergic asthmatic patients and control subjects in a dose-dependent manner in vitro. The apoptotic susceptibility of T lymphocytes to DEX and the plasma sFas concentration were significantly higher in allergic asthmatics. The ex vivo expression of Bcl-2 was significantly lower in the T lymphocytes of asthmatic patients than in those of the control subjects. However, DEX did not have any significant effect on the expression of Bcl-2 in vitro.
The T lymphocytes of asthmatic patients have higher apoptotic susceptibility to DEX treatment in vitro and a lower expression of the Bcl-2 molecule.
有研究表明，凋亡失调以及变应原反应性T淋巴细胞凋亡相关分子的表达在过敏性哮喘炎症反应的发生和维持中起关键作用。糖皮质激素是治疗过敏性炎症的有效药物。在本研究中，我们调查了地塞米松（DEX）对过敏性哮喘患者外周血T淋巴细胞凋亡、B细胞淋巴瘤（Bcl）-2表达以及可溶性Fas（sFas）的影响。
对41例过敏性哮喘患者和30例年龄及性别匹配的对照者的外周血淋巴细胞用0.1和1微摩尔/升的DEX进行处理。通过流式细胞术评估T淋巴细胞中凋亡的百分比和Bcl-2分子的表达。使用酶联免疫吸附测定法测量血浆sFas浓度。
DEX（0.1和1微摩尔/升）在体外可剂量依赖性地诱导过敏性哮喘患者和对照者的T淋巴细胞凋亡。过敏性哮喘患者T淋巴细胞对DEX的凋亡敏感性和血浆sFas浓度显著更高。哮喘患者T淋巴细胞中Bcl-2的体外表达明显低于对照者。然而，DEX在体外对Bcl-2的表达没有任何显著影响。
哮喘患者的T淋巴细胞在体外对DEX治疗具有更高的凋亡敏感性，且Bcl-2分子的表达较低。
