Qin Dong-yun, Wu Tie, Liu Jun-lin, Liang Biao
Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2007 Mar;27(3):358-61.
To study the effects of arsenic trioxide on apoptosis of peripheral T-lymphocytes from asthmatic patients and normal subjects in vitro.
The T-lymphocytes were isolated from the blood of 21 asthmatic patients and 20 healthy controls and treated with arsenic trioxide and dexamethasone. Cell apoptosis was observed by fluorescence microscope and measured with flow cytometry and Cytochrome C ELISA kit.
The T-lymphocytes from the asthmatic patients, when compared to those from of the healthy control, exhibited decelerated spontaneous apoptosis after a 24-hour incubation in vitro. Dexamethasone treatment significantly increased the percentage of apoptotic T-lymphocytes from both asthmatic patients and normal subjects in comparable magnitude. Arsenic trioxide treatment, in contrast, significantly increased the percentage of apoptotic T-lymphocytes from asthmatic patients, but slightly affected the cells from the control group.
Spontaneous apoptosis of T-lymphocytes can be decelerated in asthmatic patients, whose T-lymphocytes are more sensitive to arsenic trioxide-induced apoptosis than those of normal subjects, but the T-lymphocytes from normal subjects and asthmatic patients are equally sensitive to dexamethasone.
研究三氧化二砷对哮喘患者和正常受试者外周血T淋巴细胞体外凋亡的影响。
从21例哮喘患者和20例健康对照者的血液中分离T淋巴细胞，并用三氧化二砷和地塞米松进行处理。通过荧光显微镜观察细胞凋亡情况，并用流式细胞术和细胞色素C ELISA试剂盒进行检测。
与健康对照者相比，哮喘患者的T淋巴细胞在体外培养24小时后自发凋亡速度减慢。地塞米松处理显著增加了哮喘患者和正常受试者凋亡T淋巴细胞的百分比，且幅度相当。相比之下，三氧化二砷处理显著增加了哮喘患者凋亡T淋巴细胞的百分比，但对对照组细胞的影响较小。
哮喘患者的T淋巴细胞自发凋亡可能减慢，其T淋巴细胞对三氧化二砷诱导的凋亡比正常受试者更敏感，但正常受试者和哮喘患者的T淋巴细胞对地塞米松的敏感性相同。
