沙眼衣原体热休克调节因子HrcA的功能分析
Functional analysis of the heat shock regulator HrcA of Chlamydia trachomatis.
作者信息
Wilson Adam C, Tan Ming
机构信息
Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine, California 92697-4025, USA.
出版信息
J Bacteriol. 2002 Dec;184(23):6566-71. doi: 10.1128/JB.184.23.6566-6571.2002.
Abstract
HrcA is a regulator of bacterial heat shock gene expression that binds to a cis-acting DNA element called CIRCE. It has been proposed that HrcA and CIRCE function as a repressor-operator pair. We have purified recombinant HrcA from the pathogenic bacterium Chlamydia trachomatis and have shown that it is a DNA-binding protein that functions as a negative regulator of transcription. HrcA bound specifically to the CIRCE element in a concentration-dependent manner. HrcA repressed the in vitro transcription of a chlamydial heat shock promoter, and this repression was promoter specific. HrcA-mediated repression appears to be dependent on the topological state of the promoter, as repression on a supercoiled promoter template was greater than that on a linearized template. These results provide direct support for the role of HrcA as a transcriptional repressor in bacteria. This is the first report of the in vitro reconstitution of transcriptional regulation in Chlamydia.
摘要
HrcA是一种细菌热休克基因表达的调节因子，它与一种名为CIRCE的顺式作用DNA元件结合。有人提出HrcA和CIRCE作为阻遏物-操纵基因对发挥作用。我们从致病性细菌沙眼衣原体中纯化了重组HrcA，并表明它是一种DNA结合蛋白，作为转录的负调节因子发挥作用。HrcA以浓度依赖的方式特异性结合CIRCE元件。HrcA抑制了衣原体热休克启动子的体外转录，并且这种抑制是启动子特异性的。HrcA介导的抑制似乎依赖于启动子的拓扑状态，因为对超螺旋启动子模板的抑制大于对线性化模板的抑制。这些结果为HrcA作为细菌转录阻遏物的作用提供了直接支持。这是关于衣原体转录调控体外重建的首次报道。
相似文献
1
Functional analysis of the heat shock regulator HrcA of Chlamydia trachomatis.沙眼衣原体热休克调节因子HrcA的功能分析
J Bacteriol. 2002 Dec;184(23):6566-71. doi: 10.1128/JB.184.23.6566-6571.2002.
2
Stress response gene regulation in Chlamydia is dependent on HrcA-CIRCE interactions.衣原体中应激反应基因的调控依赖于HrcA与CIRCE的相互作用。
J Bacteriol. 2004 Jun;186(11):3384-91. doi: 10.1128/JB.186.11.3384-3391.2004.
3
A Chlamydia-specific C-terminal region of the stress response regulator HrcA modulates its repressor activity.应激反应调控蛋白 HrcA 的衣原体特异性 C 端区域调节其抑制活性。
J Bacteriol. 2011 Dec;193(23):6733-41. doi: 10.1128/JB.05792-11. Epub 2011 Sep 30.
4
Chlamydial GroEL autoregulates its own expression through direct interactions with the HrcA repressor protein.衣原体GroEL通过与HrcA阻遏蛋白的直接相互作用来自动调节其自身的表达。
J Bacteriol. 2005 Nov;187(21):7535-42. doi: 10.1128/JB.187.21.7535-7542.2005.
5
hrcA, the first gene of the Bacillus subtilis dnaK operon encodes a negative regulator of class I heat shock genes.hrcA是枯草芽孢杆菌dnaK操纵子的第一个基因，编码I类热休克基因的负调控因子。
J Bacteriol. 1996 Feb;178(4):1088-93. doi: 10.1128/jb.178.4.1088-1093.1996.
6
The GroE chaperonin machine is a major modulator of the CIRCE heat shock regulon of Bacillus subtilis.GroE伴侣蛋白机器是枯草芽孢杆菌CIRCE热休克调节子的主要调节因子。
EMBO J. 1997 Aug 1;16(15):4579-90. doi: 10.1093/emboj/16.15.4579.
7
Transcriptional organization and regulation of the dnaK and groE operons of Chlamydia trachomatis.沙眼衣原体dnaK和groE操纵子的转录组织与调控
J Bacteriol. 1996 Dec;178(23):6983-90. doi: 10.1128/jb.178.23.6983-6990.1996.
8
Identification of a Caulobacter crescentus operon encoding hrcA, involved in negatively regulating heat-inducible transcription, and the chaperone gene grpE.鉴定出一个编码hrcA的新月柄杆菌操纵子，其参与负调控热诱导转录，以及伴侣蛋白基因grpE。
J Bacteriol. 1996 Apr;178(7):1829-41. doi: 10.1128/jb.178.7.1829-1841.1996.
9
Role of HrcA and CIRCE in the heat shock regulatory network of Bradyrhizobium japonicum.HrcA和CIRCE在日本慢生根瘤菌热休克调控网络中的作用
J Bacteriol. 2000 Jan;182(1):14-22. doi: 10.1128/JB.182.1.14-22.2000.
10
Mycoplasmas regulate the expression of heat-shock protein genes through CIRCE-HrcA interactions.支原体通过CIRCE-HrcA相互作用调控热休克蛋白基因的表达。
Biochem Biophys Res Commun. 2008 Feb 29;367(1):213-8. doi: 10.1016/j.bbrc.2007.12.124. Epub 2007 Dec 28.
引用本文的文献
1
A lineage-specific heat-induced feedback loop controls HrcA to promote chlamydial fitness under stress.一种谱系特异性热诱导反馈回路控制HrcA以在应激条件下促进衣原体适应性。
bioRxiv. 2025 May 30:2025.05.30.657042. doi: 10.1101/2025.05.30.657042.
2
A conserved -specific Type III Secretion System effector linked to stress response.一种与应激反应相关的保守特异性III型分泌系统效应蛋白。
Microbiology (Reading). 2025 Apr;171(4). doi: 10.1099/mic.0.001545.
3
Expression activation of over 70% of genes during the first hour of infection.感染后第一小时内超过70%的基因表达被激活。
Infect Immun. 2024 Mar 12;92(3):e0053923. doi: 10.1128/iai.00539-23. Epub 2024 Feb 1.
4
Molecular pathogenesis of .的分子发病机制。
Front Cell Infect Microbiol. 2023 Oct 18;13:1281823. doi: 10.3389/fcimb.2023.1281823. eCollection 2023.
5
Chlamydia trachomatis RsbU Phosphatase Activity Is Inhibited by the Enolase Product, Phosphoenolpyruvate.沙眼衣原体 RsbU 磷酸酶活性受烯醇酶产物磷酸烯醇丙酮酸的抑制。
J Bacteriol. 2022 Oct 18;204(10):e0017822. doi: 10.1128/jb.00178-22. Epub 2022 Sep 19.
6
Robust Heat Shock Response in Lacking a Typical Heat Shock Sigma Factor.缺乏典型热休克西格玛因子时的强大热休克反应
Front Microbiol. 2022 Jan 3;12:812448. doi: 10.3389/fmicb.2021.812448. eCollection 2021.
7
Identification of a GrgA-Euo-HrcA Transcriptional Regulatory Network in Chlamydia.衣原体中GrgA-Euo-HrcA转录调控网络的鉴定
mSystems. 2021 Aug 31;6(4):e0073821. doi: 10.1128/mSystems.00738-21. Epub 2021 Aug 3.
8
GrgA overexpression inhibits Chlamydia trachomatis growth through sigma- and sigma-dependent mechanisms.GrgA 过表达通过 σ 和 σ 依赖性机制抑制沙眼衣原体的生长。
Microb Pathog. 2021 Jul;156:104917. doi: 10.1016/j.micpath.2021.104917. Epub 2021 May 1.
9
Sigma 54-Regulated Transcription Is Associated with Membrane Reorganization and Type III Secretion Effectors during Conversion to Infectious Forms of Chlamydia trachomatis.Sigma 54 调控的转录与沙眼衣原体转化为感染性形式过程中的膜重排和 III 型分泌效应子有关。
mBio. 2020 Sep 8;11(5):e01725-20. doi: 10.1128/mBio.01725-20.
10
The Repressor Function of the Late Regulator EUO Is Enhanced by the Plasmid-Encoded Protein Pgp4.晚期调控因子 EUO 的抑制功能可被质粒编码蛋白 Pgp4 增强。
J Bacteriol. 2020 Mar 26;202(8). doi: 10.1128/JB.00793-19.
本文引用的文献
1
Renaturation of Bacillus thermoglucosidasius HrcA repressor by DNA and thermostability of the HrcA-DNA complex in vitro.嗜热葡萄糖苷芽孢杆菌HrcA阻遏蛋白的DNA复性及HrcA-DNA复合物在体外的热稳定性
J Bacteriol. 2001 Jan;183(1):155-61. doi: 10.1128/JB.183.1.155-161.2001.
2
Three temporal classes of gene expression during the Chlamydia trachomatis developmental cycle.沙眼衣原体发育周期中的三个时间类别的基因表达。
Mol Microbiol. 2000 Aug;37(4):913-25. doi: 10.1046/j.1365-2958.2000.02057.x.
3
A positive cis-acting DNA element is required for high-level transcription in Chlamydia.衣原体中的高水平转录需要一个正向顺式作用DNA元件。
J Bacteriol. 2000 Sep;182(18):5167-71. doi: 10.1128/JB.182.18.5167-5171.2000.
4
Expression of the transcripts of the sigma factors and putative sigma factor regulators of Chlamydia trachomatis L2.沙眼衣原体L2的σ因子及假定的σ因子调节因子转录本的表达
Gene. 2000 Apr 18;247(1-2):209-14. doi: 10.1016/s0378-1119(00)00094-9.
5
Escherichia coli response to hydrogen peroxide: a role for DNA supercoiling, topoisomerase I and Fis.大肠杆菌对过氧化氢的反应：DNA超螺旋、拓扑异构酶I和Fis的作用
Mol Microbiol. 2000 Mar;35(6):1413-20. doi: 10.1046/j.1365-2958.2000.01805.x.
6
Role of HrcA and CIRCE in the heat shock regulatory network of Bradyrhizobium japonicum.HrcA和CIRCE在日本慢生根瘤菌热休克调控网络中的作用
J Bacteriol. 2000 Jan;182(1):14-22. doi: 10.1128/JB.182.1.14-22.2000.
7
Development of a quantitative gene expression assay for Chlamydia trachomatis identified temporal expression of sigma factors.沙眼衣原体定量基因表达检测方法的开发确定了σ因子的时序表达。
FEBS Lett. 1999 Sep 24;458(3):354-8. doi: 10.1016/s0014-5793(99)01182-5.
8
Chlamydial heat shock proteins and disease pathology: new paradigms for old problems?衣原体热休克蛋白与疾病病理学：老问题的新范式？
Infect Dis Obstet Gynecol. 1999;7(1-2):64-71. doi: 10.1155/S1064744999000137.
9
Comparative genomes of Chlamydia pneumoniae and C. trachomatis.肺炎衣原体和沙眼衣原体的比较基因组
Nat Genet. 1999 Apr;21(4):385-9. doi: 10.1038/7716.
10
Negative regulation of bacterial heat shock genes.细菌热休克基因的负调控
Mol Microbiol. 1999 Jan;31(1):1-8. doi: 10.1046/j.1365-2958.1999.01166.x.
Zotero 插件