大肠杆菌对4,5-二羟基-2-环戊烯-1-酮处理反应的表达谱的DNA微阵列分析
DNA microarray analysis of the expression profile of Escherichia coli in response to treatment with 4,5-dihydroxy-2-cyclopenten-1-one.
作者信息
Phadtare Sangita, Kato Ikunoshin, Inouye Masayori
机构信息
Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.
出版信息
J Bacteriol. 2002 Dec;184(23):6725-9. doi: 10.1128/JB.184.23.6725-6729.2002.
Abstract
We carried out DNA microarray-based global transcript profiling of Escherichia coli in response to 4,5-dihydroxy-2-cyclopenten-1-one to explore the manifestation of its antibacterial activity. We show that it has widespread effects in E. coli affecting genes encoding proteins involved in cell metabolism and membrane synthesis and functions. Genes belonging to the regulon involved in synthesis of Cys are upregulated. In addition, rpoS and RpoS-regulated genes responding to various stresses and a number of genes responding to oxidative stress are upregulated.
摘要
我们利用基于DNA微阵列的技术对大肠杆菌进行了全转录组分析，以探究其对4,5 - 二羟基 - 2 - 环戊烯 - 1 - 酮的抗菌活性表现。我们发现它对大肠杆菌具有广泛影响，涉及影响编码参与细胞代谢、膜合成及功能的蛋白质的基因。属于参与半胱氨酸合成的调节子的基因上调。此外，对各种应激作出反应的rpoS及RpoS调节基因以及一些对氧化应激作出反应的基因也上调。
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