Rozen Yael, Larossa Robert A, Templeton Lori J, Smulski Dana R, Belkin Shimshon
Fredy and Nadine Herrmann Graduate School of Applied Science, Hebrew University, Jerusalem, Israel.
Antonie Van Leeuwenhoek. 2002 Aug;81(1-4):15-25. doi: 10.1023/a:1020500821856.
Gene expression of Escherichia coli cells exposed to seawater for 20 h was compared to that of exponentially growing cells (mops-glucose 0.2%) using DNA microarray technology. The expression of most (ca. 3,000) of the 4,228 open reading frames on the microarray remained unchanged; the relative expression of about 320 genes decreased in seawater, whereas that of ca. one fourth (937) increased. Clearly coherent expression patterns were observed for several functional gene groups. Induced genes were numerous in groups specifying the degradation of small molecules (carbon compounds, amino acids and fatty acids), energy metabolism (aerobic and anaerobic respiration, pyruvate dehydrogenase and TCA cycle), chemotaxis and mobility, flagella biosynthesis, surface structures and phage related functions. Repressed genes were clustered in two groups, cell division and nucleotides biosynthesis, indicating a cessation of growth.
使用DNA微阵列技术，将暴露于海水中20小时的大肠杆菌细胞的基因表达与指数生长细胞（mops - 葡萄糖0.2%）的基因表达进行了比较。微阵列上4228个开放阅读框中的大多数（约3000个）表达保持不变；约320个基因的相对表达在海水中下降，而约四分之一（937个）基因的相对表达增加。在几个功能基因组中观察到明显一致的表达模式。在指定小分子（碳化合物、氨基酸和脂肪酸）降解、能量代谢（有氧和无氧呼吸、丙酮酸脱氢酶和三羧酸循环）、趋化性和运动性、鞭毛生物合成、表面结构和噬菌体相关功能的组中，诱导基因数量众多。受抑制的基因聚集在两组中，即细胞分裂和核苷酸生物合成，表明生长停止。
