Jewell Scott D, Srinivasan Mythily, McCart Linda M, Williams Nita, Grizzle William H, LiVolsi Virginia, MacLennan Greg, Sedmak Daniel D
Department of Pathology, Ohio State University, Columbus 43210, USA.
Am J Clin Pathol. 2002 Nov;118(5):733-41. doi: 10.1309/VPQL-RT21-X7YH-XDXK.
The scientific usefulness of the data obtained from tissue analysis is related to specimen quality, which may be affected by conditions that may contribute to the degradation of the specimen before processing and analysis. We determined the usability of nucleic acids extracted from banked human tissues for further molecular analyses. We assayed 151 tissue specimens, storedfor various times at 4 divisions of the Cooperative Human Tissue Network, National Cancer Institute, Bethesda, MD, for DNA and RNA degradation. Simple electrophoresis, polymerase chain reaction (PCR), reverse-transcriptase (RT)-PCR, and Northern blot analysis were compared to determine the optimal quality control procedure. In addition, a time course degradation procedure was performed on human lung tissue. Gel electrophoresis was as informative as PCR, RT-PCR, and Northern blot analysis in determining the molecular usefulness of the human tissues. Overall, 80% of the stored human tissues had good-quality DNA, and 60% had good-quality RNA. Electrophoresis procedures for DNA and RNA offer a quick and valuable measure of the molecular quality of stored human tissues. The DNA and RNA degradation of one tissue type (lung) was stable for both nucleic acids for up to 5 hours after excision.
从组织分析中获得的数据的科学效用与标本质量相关，标本质量可能会受到一些条件的影响，这些条件可能会在处理和分析之前导致标本降解。我们确定了从储存的人体组织中提取的核酸用于进一步分子分析的可用性。我们检测了151个组织标本，这些标本在位于马里兰州贝塞斯达的国家癌症研究所合作人体组织网络的4个部门中储存了不同时间，以检测DNA和RNA的降解情况。比较了简单电泳、聚合酶链反应（PCR）、逆转录酶（RT）-PCR和Northern印迹分析，以确定最佳质量控制程序。此外，对人肺组织进行了时间进程降解程序。在确定人体组织的分子效用方面，凝胶电泳与PCR、RT-PCR和Northern印迹分析一样具有信息价值。总体而言，80%的储存人体组织具有高质量的DNA，60%具有高质量的RNA。DNA和RNA的电泳程序为储存人体组织的分子质量提供了快速且有价值的衡量方法。一种组织类型（肺）的DNA和RNA降解在切除后长达5小时内对两种核酸都是稳定的。
