González Mónica, Méndez Jesús, Carnero Aurelio, Lobo M Gloria, Afonso Ana
Plant Physiology Laboratory, Instituto Canario de Investigaciones Agrarias, 38006 La Laguna, Spain.
J Agric Food Chem. 2002 Nov 20;50(24):6968-74. doi: 10.1021/jf025756r.
A simple method was developed for the extraction and determination of color pigments in cochineals (Dactylopius coccus Costa). The procedure was based on the solvent extraction of pigments in insect samples using methanol:water (65:35, v:v) as extractant. Two-level factorial design was used in order to optimize the solvent extraction parameters: temperature, time, methanol concentration in the extractant mixture, and the number of extractions. The results suggest that the number of extractions is statistically the most significant factor. The separation and determination of the pigments was carried out by high-performance liquid chromatography with UV-visible detection. Because the absorption spectra of different pigments are different in the visible region, it is convenient to use a diode array detector to obtain chromatographic profiles that allow for the characterization of the extracted pigments.
开发了一种用于提取和测定胭脂虫（Dactylopius coccus Costa）中色素的简单方法。该方法基于使用甲醇：水（65：35，v：v）作为萃取剂对昆虫样品中的色素进行溶剂萃取。采用二水平析因设计以优化溶剂萃取参数：温度、时间、萃取剂混合物中的甲醇浓度和萃取次数。结果表明，萃取次数在统计学上是最显著的因素。色素的分离和测定通过配备紫外-可见检测器的高效液相色谱法进行。由于不同色素在可见光区域的吸收光谱不同，使用二极管阵列检测器获得色谱图以表征萃取的色素很方便。
