Chroma Ludmila, Macek Tomas, Demnerova Katerina, Macková Martina
Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, ICT Prague, Czech Republic.
Chemosphere. 2002 Nov;49(7):739-48. doi: 10.1016/s0045-6535(02)00397-1.
An extracellular H2O2-requiring Remazol Brilliant Blue R (RBBR) decolorizing enzyme activity was detected after cultivation of cells of various plant species both in liquid medium and when growing on agar plates containing RBBR. Level of the enzyme activity was compared with the ability to metabolize polychlorinated biphenyls (PCBs). The ability to decolorize RBBR was tested in the presence and absence of PCBs. The cultures with high PCB-transforming activity proved to exhibit RBBR oxidase much more resistant towards the influence of PCBs. In addition low activities of lignin peroxidase (LiP) and manganese dependent peroxidase (MnP) were detected in medium and in plant cells. No correlation of MnP and LiP activities with PCB degradation could be found. The RBBR decolorization could be used as a rough screening method for plant cultures able to metabolize PCBs.
在多种植物细胞于液体培养基中培养以及在含有活性艳蓝R（RBBR）的琼脂平板上生长后，检测到一种需要细胞外过氧化氢的RBBR脱色酶活性。将该酶活性水平与多氯联苯（PCBs）代谢能力进行了比较。在有和没有PCBs存在的情况下测试了RBBR脱色能力。具有高PCB转化活性的培养物被证明对PCBs的影响表现出更强的RBBR氧化酶抗性。此外，在培养基和植物细胞中检测到低水平的木质素过氧化物酶（LiP）和锰依赖过氧化物酶（MnP）。未发现MnP和LiP活性与PCB降解之间存在相关性。RBBR脱色可作为一种对能够代谢PCBs的植物培养物进行初步筛选的方法。
