Dandekar S P, Nadkarni G D, Kulkarni V S, Punekar S
Department of Biochemistry, Seth G. S. Medical College, Parel, Mumbai-400 012, India.
J Postgrad Med. 2002 Jul-Sep;48(3):186-89; discussion 189-90.
Mammalian spermatozoa are rich in polyunsaturated fatty acids and are very susceptible to attack by reactive oxygen species (ROS) and membrane lipid peroxide ion. Normally a balance is maintained between the amount of ROS produced and that scavenged. Cellular damage arises when this equilibrium is disturbed. A shift in the levels of ROS towards pro-oxidants in semen and vaginal secretions can induce an oxidative stress on spermatozoa. The aim was to study lipid peroxidation and antioxidant enzymes such as catalase, glutathione peroxidase and superoxide dismutase (SOD) and to correlate the same, with the 'water test', in male infertility.
Experimental study.
Ejaculates from a total of 83 infertile and fertile healthy individuals were obtained. Lipid peroxidation and antioxidant enzyme levels were studied and correlated with water test.
The results indicate that (i) the antioxidant enzyme catalase showed no significant changes in the various pathological samples, (ii) antioxidant enzymes SOD and glutathione peroxidase correlate positively with asthenozoospermic samples and (iii) the degree of lipid peroxidation also correlates positively with the poorly swollen sperm tails. The increase in SOD and glutathione peroxidase values, in the pathological cases represents an attempt made to overcome the reactive oxygen species.
Water test could be used as a preliminary marker test for sperm tail damage by reactive oxygen species, since it correlates very well with lipid peroxidation and antioxidant enzymes.
哺乳动物精子富含多不饱和脂肪酸，极易受到活性氧（ROS）和膜脂质过氧化物离子的攻击。正常情况下，ROS的产生量与清除量保持平衡。当这种平衡被打破时，细胞就会受损。精液和阴道分泌物中ROS水平向促氧化剂方向的转变会对精子产生氧化应激。目的是研究脂质过氧化以及过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶（SOD）等抗氧化酶，并将其与男性不育症中的“水试验”相关联。
实验研究。
共采集了83名不育和健康可育个体的精液。研究脂质过氧化和抗氧化酶水平，并将其与水试验相关联。
结果表明，（i）抗氧化酶过氧化氢酶在各种病理样本中无显著变化，（ii）抗氧化酶SOD和谷胱甘肽过氧化物酶与弱精子症样本呈正相关，（iii）脂质过氧化程度也与精子尾部肿胀不良呈正相关。病理情况下SOD和谷胱甘肽过氧化物酶值的增加代表了克服活性氧的一种尝试。
水试验可作为活性氧对精子尾部损伤的初步标记试验，因为它与脂质过氧化和抗氧化酶相关性很好。
