Ghiasvand Tayebeh, Goodarzi Mohammad Taghi, Shafiee Gholamreza, Zamani Alireza, Karimi Jamshid, Ghorbani Marzieh, Amiri Iraj
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan Iran.
Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Int J Reprod Biomed. 2018 Feb;16(2):93-100.
Neopterin is a significant and sensitive marker in estimating the activity of cellular immune system. Oxidative stress plays a role in the etiology of male infertility. Increased reactive oxygen species is accompanied with increase in neopterin level. Hence neopterin may be involved in male infertility.
The objective of this case-control study was to determine neopterin level in idiopathic infertile and normospermic men; furthermore, to identify its relationship with oxidative stress markers including total oxidant, malondialdehyde, sperm DNA fragmentation, and total antioxidant capacity of seminal plasma.
Forty seven infertile and forty three normospermic males were selected according to WHO criteria. Their semen and blood samples were taken; subsequently, the levels of neopterin, total oxidant, total antioxidant, malondialdehyde, and sperm DNA fragmentation were measured.
The levels of neopterin, total oxidant, and malondialdehyde in seminal plasma of infertile males were significantly higher than those of normospermic group (p=0.038, 0.018, and 0.028, respectively). Furthermore, sperm DNA fragmentation in infertile men was higher than that of control group (p<0.001). Moreover, total antioxidant capacity of seminal plasma in infertile males was significantly lower than that of normospermic subjects (p=0.002). No significant difference was observed in serum neopterin, total oxidant, and malondialdehyde between the infertile and normospermic groups.
The significant inverse correlation between seminal plasma neopterin and total antioxidant in the infertile males supports a possible role of neopterin in male infertility. Neopterin can be suggested as a marker in monitoring and diagnosis of idiopathic male infertility.
蝶呤是评估细胞免疫系统活性的重要且敏感的标志物。氧化应激在男性不育症的病因中起作用。活性氧增加伴随着蝶呤水平升高。因此，蝶呤可能与男性不育症有关。
本病例对照研究的目的是测定特发性不育男性和精液正常男性的蝶呤水平；此外，确定其与氧化应激标志物的关系，包括总氧化剂、丙二醛、精子DNA碎片以及精浆总抗氧化能力。
根据世界卫生组织标准选择47名不育男性和43名精液正常男性。采集他们的精液和血液样本；随后，测定蝶呤、总氧化剂、总抗氧化剂、丙二醛和精子DNA碎片的水平。
不育男性精浆中蝶呤、总氧化剂和丙二醛水平显著高于精液正常组（分别为p = 0.038、0.018和0.028）。此外，不育男性的精子DNA碎片高于对照组（p < 0.001）。而且，不育男性精浆的总抗氧化能力显著低于精液正常者（p = 0.002）。不育组和精液正常组之间血清蝶呤、总氧化剂和丙二醛无显著差异。
不育男性精浆中蝶呤与总抗氧化剂之间的显著负相关支持蝶呤在男性不育症中可能发挥的作用。蝶呤可作为监测和诊断特发性男性不育症的标志物。
