千根癀的抗氧化和抗病毒活性

Antioxidant and antiviral activities of Euphorbia thymifolia L.

作者信息

Lin Chun-Ching, Cheng Hua-Yew, Yang Chien-Min, Lin Ta-Chen

机构信息

Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.

出版信息

J Biomed Sci. 2002 Nov-Dec;9(6 Pt 2):656-64. doi: 10.1159/000067281.

DOI:10.1159/000067281

PMID:12432232

Abstract

The antioxidant and antiviral activities of Euphorbia thymifolia L. (Euphorbiaceae) were investigated in this study. The results showed that all of the fractions (MeOH, CHCl(3), EtOAc, n-butanol and water) and pure compounds (3-O-galloyl-4,6-(S)-HHDP-D-glucose, rugosin B and 1,3,4,6-tetra-O-galloyl-K-beta-D-glucose)tested possessed antioxidant activities, with the exception of the organic aqueous fraction in the anti-lipid and anti-superoxide formation assays. The range of IC(50) of anti-lipid formation, anti-superoxide formation and free radical scavenging assays for all fractions and pure compounds were 2.81-7.63, 0.03-2.18 and 0.013-2.878 mg/ml, respectively. Electron spin resonance studies showed that water extract and pure compounds of E. thymifolia exhibited superoxide radical and hydroxyl radical scavenging activities. Besides antioxidant activities, 3-O-galloyl-4,6-(S)-HHDP-D-glucose and EtOAc fraction also showed anti-HSV-2 activity. Thus, E. thymifolia was concluded to possess antioxidant and anti-HSV-2 activities.

摘要

本研究对千根癀（大戟科）的抗氧化和抗病毒活性进行了研究。结果表明，除了在抗脂质和抗超氧化物形成试验中的有机水相部分外，所有测试的馏分（甲醇、氯仿、乙酸乙酯、正丁醇和水）以及纯化合物（3 - O - 没食子酰基 - 4,6 - (S) - HHDP - D - 葡萄糖、rugosin B和1,3,4,6 - 四 - O - 没食子酰基 - K - β - D - 葡萄糖）均具有抗氧化活性。所有馏分和纯化合物在抗脂质形成、抗超氧化物形成和自由基清除试验中的IC50范围分别为2.81 - 7.63、0.03 - 2.18和0.013 - 2.878 mg/ml。电子自旋共振研究表明，千根癀的水提取物和纯化合物具有超氧化物自由基和羟基自由基清除活性。除抗氧化活性外，3 - O - 没食子酰基 - 4,6 - (S) - HHDP - D - 葡萄糖和乙酸乙酯馏分还表现出抗单纯疱疹病毒2型（HSV - 2）的活性。因此，得出千根癀具有抗氧化和抗HSV - 2活性的结论。