Medical College, Shantou University, Guangdong, China 515031.
Biol Res. 2009;42(3):351-6. Epub 2009 Nov 2.
Schisandra propinqua (Wall.) Baill.(Schisandraceae) is widely used as a Chinese folk medicine. In this study, activity-guided fractionation of the ethanol extract from the stem of Schisandra propinqua led to the isolation of four extracts. Subsequently, a neolignan 4,4-di(4-hydroxy-3-methoxyphenly)-2,3-dimethylbutanol was isolated from the EtOAc part of the stem of Schisandra propinqua, the free radical scavenging activities of which were researched in vitro. The present work demonstrated that extracts and pure compound possessed scavenging activities to DPPH, superoxide anions and hydroxy radical, and could depress lipid peroxidation reaction induced by oxygen radical produced by the Fe2+/cysteine system in vitro. This suggests that the traditional application of Schisandra propinqua in China may be related to its antioxidant activities, and the EtOAc part of the stems of Schisandra propinqua can be utilized as an effective source of antioxidants.
北五味子（五味子科）在民间被广泛用作中药。本研究采用活性导向分离法从北五味子茎的乙醇提取物中分离得到四个部位。随后，从北五味子茎的乙酸乙酯部分中分离得到一个新木脂素 4,4-二（4-羟基-3-甲氧基苯基）-2,3-二甲基丁醇，研究了其体外自由基清除活性。本工作表明，提取物和纯化合物均具有清除 DPPH、超氧阴离子自由基和羟自由基的活性，并且能够抑制 Fe2+/半胱氨酸体系产生的氧自由基诱导的脂质过氧化反应。这表明，北五味子在中国的传统应用可能与其抗氧化活性有关，北五味子茎的乙酸乙酯部分可作为有效的抗氧化剂来源。
