Elkahloun Abdel G, Gaudet Justin, Robinson Gregory S, Sgroi Dennis C
Cancer Genetics Branch, NHGRI-NIH, Bethesda, MD 20892, USA.
Cancer Biol Ther. 2002 Jul-Aug;1(4):354-8.
The simultaneous development of laser capture microdissection (LCM) and high-throughput mRNA analysis platforms has provided a significant technological advance in the world of cancer biology. The combination of such technologies provides a unique and powerful opportunity to directly assess the in situ molecular genetic events that are associated with initiation and progression of human malignancies. Despite these technological advances, the integration of LCM with high-throughput gene expression analysis has been met with various challenges. The goal of this review is to highlight some of the obstacles that we have faced and continue to face as we try to optimally apply LCM to in situ gene expression analysis.
激光捕获显微切割技术（LCM）与高通量mRNA分析平台的同步发展，为癌症生物学领域带来了重大的技术进步。这些技术的结合提供了一个独特而强大的机会，可直接评估与人类恶性肿瘤发生和发展相关的原位分子遗传事件。尽管有这些技术进步，但LCM与高通量基因表达分析的整合仍面临各种挑战。本综述的目的是强调在我们试图将LCM最佳地应用于原位基因表达分析时已经面临并将继续面临的一些障碍。
