Farragher Susan M, Tanney Austin, Kennedy Richard D, Paul Harkin D
Almac Diagnostics, Craigavon, 19 Seagoe Industrial Estate, Armagh BT635QD, UK.
Histochem Cell Biol. 2008 Sep;130(3):435-45. doi: 10.1007/s00418-008-0479-7. Epub 2008 Aug 5.
Formalin fixation and paraffin embedding (FFPE) is the most commonly used method worldwide for tissue storage. This method preserves the tissue integrity but causes extensive damage to nucleic acids stored within the tissue. As methods for measuring gene expression such as RT-PCR and microarray are adopted into clinical practice there is an increasing necessity to access the wealth of information locked in the Formalin fixation and paraffin embedding archives. This paper reviews the progress in this field and discusses the unique opportunities that exist for the application of these techniques in the development of personalized medicine.
福尔马林固定和石蜡包埋（FFPE）是全球最常用的组织储存方法。这种方法能保持组织完整性，但会对组织内储存的核酸造成广泛损伤。随着诸如逆转录聚合酶链反应（RT-PCR）和微阵列等基因表达测量方法应用于临床实践，获取福尔马林固定和石蜡包埋档案中所蕴含的丰富信息的需求日益增加。本文综述了该领域的进展，并讨论了这些技术在个性化医疗发展中应用所存在的独特机遇。
