毛细管电泳双输入电容耦合非接触电导检测的模拟与实验研究

Simulation and Experimental Study on Doubled-Input Capacitively Coupled Contactless Conductivity Detection of Capillary Electrophoresis.

作者信息

Wang Chunling, Xing Haoyang, Zheng Baozhan, Yuan Hongyan, Xiao Dan

机构信息

College of Chemical Engineering, Sichuan University, Chengdu, 610064, People's Republic of China.

College of Physical Science and Technology, Sichuan University, Chengdu, 610065, People's Republic of China.

出版信息

Sci Rep. 2020 May 14;10(1):7944. doi: 10.1038/s41598-020-64896-3.

DOI:10.1038/s41598-020-64896-3

PMID:32409736

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7224287/

Abstract

In this contribution, we optimize the structure of double-input capacitively coupled contactless conductivity detector (DICD) that proposed before by our group and successfully applied it in the capillary electrophoresis of inorganic ion analysis. Furthermore, we present the detail theoretical analysis and simulation to exploring the working mechanism of DICD. Compared with CD, under identical experimental conditions and by using the same current-to-voltage converter, both the theoretical and experimental results suggest that the effectiveness and feasibility of DICD. The improved DICD diminished the baseline drift effects in CD, provides lower noise, higher sensitivity and notably stable baseline. The LODs of DICD are 1.0 μM for K and 1.5 μM for Li (S/N = 3). DICD provides a better linear relationship (R = 0.997 and 0.998 for K and Li, respectively) with the range of 2.0 μM ~ 2.5 mM.

摘要

在本论文中，我们优化了本课题组之前提出的双输入电容耦合非接触式电导检测器（DICD）的结构，并成功将其应用于无机离子分析的毛细管电泳中。此外，我们还进行了详细的理论分析和模拟，以探究DICD的工作机制。与电导检测器（CD）相比，在相同的实验条件下，使用相同的电流-电压转换器，理论和实验结果均表明DICD的有效性和可行性。改进后的DICD减少了CD中的基线漂移效应，具有更低的噪声、更高的灵敏度和显著更稳定的基线。DICD对K的检测限为1.0 μM，对Li的检测限为1.5 μM（信噪比S/N = 3）。DICD在2.0 μM至2.5 mM的范围内呈现出更好的线性关系（K和Li的相关系数R分别为0.997和0.998）。