Orelio Claudia, Dzierzak Elaine
Department of Cell Biology and Genetics, Erasmus University Medical Center, Rotterdam, The Netherlands.
Blood. 2003 Mar 15;101(6):2246-9. doi: 10.1182/blood-2002-07-2260. Epub 2002 Nov 14.
The first adult-repopulating hematopoietic stem cells (HSCs) emerge in the mouse aorta-gonad-mesonephros (AGM) region at embryonic day 10.5 prior to their appearance in the yolk sac and fetal liver. Although several genes are implicated in the regulation of HSCs, there are gaps in our understanding of the processes taking place in the AGM at the time of HSC emergence. To identify genes involved in AGM HSC emergence, we performed differential display reverse transcriptase-polymerase chain reaction (DD RT-PCR). Differentially expressed genes included beta-catenin and homologs of human TM9SF2 and TAB2. We characterized the expression pattern of Wnt/beta-catenin signaling, mTM9SF2, and mTAB2 in the embryo and adult. Interestingly, the expression of mouse TAB2 (mTAB2) in the E11 dorsal aorta endothelium suggests a role for mTAB2 in HSC emergence and/or regulation. The identification of differentially expressed genes in the AGM region should yield further insights into the development of this tissue and into the emergence and regulation of HSCs.
第一批能够在成年期重新填充的造血干细胞（HSCs）于胚胎第10.5天在小鼠的主动脉-性腺-中肾（AGM）区域出现，早于它们在卵黄囊和胎肝中的出现时间。尽管有几个基因参与造血干细胞的调控，但我们对造血干细胞出现时AGM区域发生的过程的理解仍存在空白。为了鉴定参与AGM区域造血干细胞出现的基因，我们进行了差异显示逆转录聚合酶链反应（DD RT-PCR）。差异表达的基因包括β-连环蛋白以及人类TM9SF2和TAB2的同源物。我们对Wnt/β-连环蛋白信号通路、mTM9SF2和mTAB2在胚胎和成年个体中的表达模式进行了表征。有趣的是，小鼠TAB2（mTAB2）在胚胎第11天背主动脉内皮中的表达表明mTAB2在造血干细胞的出现和/或调控中发挥作用。在AGM区域鉴定差异表达基因应能进一步深入了解该组织的发育以及造血干细胞的出现和调控。
