Steger David J, Haswell Elizabeth S, Miller Aimee L, Wente Susan R, O'Shea Erin K
Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143-0448, USA.
Science. 2003 Jan 3;299(5603):114-6. doi: 10.1126/science.1078062. Epub 2002 Nov 14.
Chromatin remodeling is required for efficient transcription of eukaryotic genes. In a genetic selection for budding yeast mutants that were defective in induction of the phosphate-responsive PHO5 gene, we identified mutations in ARG82/IPK2, which encodes a nuclear inositol polyphosphate kinase. In arg82 mutant strains, remodeling of PHO5 promoter chromatin is impaired, and the adenosine triphosphate-dependent chromatin-remodeling complexes SWI/SNF and INO80 are not efficiently recruited to phosphate-responsive promoters. These results suggest a role for the small molecule inositol polyphosphate in the regulation of chromatin remodeling and transcription.
染色质重塑是真核基因高效转录所必需的。在对出芽酵母突变体进行的遗传筛选中，这些突变体在诱导磷酸盐应答性PHO5基因方面存在缺陷，我们在ARG82/IPK2中发现了突变，该基因编码一种核肌醇多磷酸激酶。在arg82突变体菌株中，PHO5启动子染色质的重塑受损，并且三磷酸腺苷依赖性染色质重塑复合物SWI/SNF和INO80不能有效地募集到磷酸盐应答性启动子上。这些结果表明小分子肌醇多磷酸在染色质重塑和转录调控中发挥作用。
