Ponti Donatella, Mangoni M Luisa, Mignogna Giuseppina, Simmaco Maurizio, Barra Donatella
Dipartimento di Scienze Biochimiche 'A. Rossi Fanelli'; CNR, Centro di Biologia Molecolare, Università La Sapienza, 00185 Roma, Italy.
Biochem J. 2003 Feb 15;370(Pt 1):121-7. doi: 10.1042/BJ20021444.
Esculentin-1 is a 46-residue antimicrobial peptide present in skin secretions of Rana esculenta. It is effective against a wide variety of micro-organisms, including plant pathogens with negligible effects on eukaryotic cells. As a possible approach to enhance plant resistance, a DNA coding for esculentin-1, with the substitution Met-28Leu, was fused at the C-terminal end of the leader sequence of endopolygalacturonase-inhibiting protein, under the control of the cauliflower mosaic virus 35S promoter region, and introduced into Nicotiana tabacum. The antimicrobial peptide was isolated from the intercellular fluids of healthy leaves of transgenic plants, suggesting that it was properly processed, secreted outside cells and accumulated in the intercellular spaces. The morphology of transgenic plants was unaffected. Challenging these plants with bacterial or fungal phytopathogens demonstrated enhanced resistance up to the second generation. Moreover, transgenic plants displayed insecticidal properties.
埃斯库林-1是一种存在于食用蛙皮肤分泌物中的46个氨基酸残基的抗菌肽。它对多种微生物有效，包括植物病原体，而对真核细胞的影响可忽略不计。作为增强植物抗性的一种可能方法，编码埃斯库林-1（Met-28Leu被替换）的DNA在花椰菜花叶病毒35S启动子区域的控制下，融合在内聚半乳糖醛酸酶抑制蛋白前导序列的C末端，并导入烟草。从转基因植物健康叶片的细胞间液中分离出抗菌肽，这表明它经过了正确的加工，分泌到细胞外并积累在细胞间隙中。转基因植物的形态未受影响。用细菌或真菌植物病原体挑战这些植物，结果表明其抗性增强，直至第二代。此外，转基因植物还表现出杀虫特性。
