Cho Jaeyoung, Lee Sang-Hyun, Seo Je Hoon, Kim Hee-Sung, Ahn Jung-Gil, Kim Sung-Soo, Yim Sung-Vin, Song Dong-Keun, Cho Sa Sun
Department of Anatomy, Seoul National University College of Medicine and Institute of Neuroscience, Medical research Center, Seoul National University, Yongon-dong 28, Chongno-gu, Seoul 110-799, South Korea.
Neurosci Lett. 2002 Dec 13;334(2):131-4. doi: 10.1016/s0304-3940(02)01122-9.
A phosphatase and tensin homolog (PTEN) has been known to play multiple biological roles. However, role of PTEN in astrocyte activation is not clear yet. In the present study, the expression pattern of PTEN in the process of reactive gliosis was immunohistochemically examined in intracerebroventricular (i.c.v.) injected kainic acid mouse hippocampus. Mice were grouped into three; 30 min, 1 day and 7 days after kainic acid i.c.v. injection. Thirty minutes after kainic acid i.c.v. injection, astrocytes were activated and PTEN was weakly expressed in immature astrocytes. Seven days after kainic acid i.c.v. injection, PTEN expression was decreased in highly activated astrocytes showing extensively spindled shape. Immunofluorescence double labeling experiment showed that PTEN was expressed in glial fibrillary acidic protein-positive astrocytes. These findings suggest that PTEN might have a role in early stage of reactive astrogliosis in vivo.
已知磷酸酶和张力蛋白同源物（PTEN）发挥多种生物学作用。然而，PTEN在星形胶质细胞激活中的作用尚不清楚。在本研究中，采用免疫组织化学方法检测了脑室内注射海藻酸的小鼠海马中反应性胶质增生过程中PTEN的表达模式。将小鼠分为三组，分别在脑室内注射海藻酸后30分钟、1天和7天。脑室内注射海藻酸30分钟后，星形胶质细胞被激活，PTEN在未成熟星形胶质细胞中弱表达。脑室内注射海藻酸7天后，在高度激活且呈现广泛梭形的星形胶质细胞中PTEN表达降低。免疫荧光双标记实验表明，PTEN在胶质纤维酸性蛋白阳性的星形胶质细胞中表达。这些发现提示PTEN可能在体内反应性星形胶质增生的早期阶段发挥作用。
