Jeon Gye Sun, Shin Dong Hoon, Cho Sa Sun
Department of Anatomy, Seoul National University, College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea.
Neurosci Lett. 2004 Apr 22;360(1-2):13-6. doi: 10.1016/j.neulet.2004.01.017.
The transcription factor c-myb is known to play an important role in the regulation of cellular proliferation and differentiation. Recently, the constitutive and aberrant expression of c-myb in the normal and Cu/Zn SOD mutant mouse brain was reported. However, the expression of c-myb in the process of reactive gliosis is not known yet. Here we report the delayed and protracted induction of c-myb in the brain of mice following kainic acid (KA) induced seizure. Our western blot analysis revealed that the amount of c-myb was dramatically increased in the brain 3 days after KA treatment. The induction of c-myb was sustained for more than 7 days after KA treatment. The c-myb immunoreactivity (IR) was restricted to neurons of the hippocampus in control mice. Three days after KA treatment, a strong c-myb IR was found in reactive astrocytes in the whole areas of the CA3 region. Thereafter, c-myb IR astrocytes were gradually concentrated around the CA3 region undergoing selective neuronal loss. A few c-myb IR astrocytes were continuously persisted in the CA3 region 14 days after KA treatment. These findings suggest a role of c-myb signal pathway in reactive gliosis in mice with KA induced seizure.
已知转录因子c-myb在细胞增殖和分化的调控中发挥重要作用。最近，有报道称c-myb在正常小鼠和铜锌超氧化物歧化酶（Cu/Zn SOD）突变小鼠大脑中存在组成型和异常表达。然而，c-myb在反应性胶质增生过程中的表达情况尚不清楚。在此，我们报道了在 kainic acid（KA）诱导癫痫发作后，小鼠大脑中c-myb的诱导延迟且持续时间延长。我们的蛋白质印迹分析显示，KA处理3天后，大脑中c-myb的量显著增加。KA处理后，c-myb的诱导持续超过7天。在对照小鼠中，c-myb免疫反应性（IR）局限于海马神经元。KA处理3天后，在CA3区整个区域的反应性星形胶质细胞中发现强烈的c-myb IR。此后，c-myb IR星形胶质细胞逐渐集中在经历选择性神经元丢失的CA3区周围。KA处理14天后，少数c-myb IR星形胶质细胞持续存在于CA3区。这些发现表明c-myb信号通路在KA诱导癫痫发作的小鼠反应性胶质增生中发挥作用。
