牛、兔、大鼠和绵羊肺表面活性物质的特性研究
Characterization of pulmonary surfactant from ox, rabbit, rat and sheep.
作者信息
Harwood J L, Desai R, Hext P, Tetley T, Richards R
出版信息
Biochem J. 1975 Dec;151(3):707-14. doi: 10.1042/bj1510707.
Abstract
- Pulmonary surfactants from ox, rabbit, rat and sheep were isolated and analysed. 2. All preparations had a high anenoic phosphatidylcholine content and would produce stable surface tensions of 0.01 Nm-1 or less. 3. Protein content was 8-18% of the dry weights. A number of proteins were observed; their overall composition were high in hydrophobic amino acid residues. 4. Lipid content varied from 79% (ox) to 90% (rabbit) with phosphatidylcholine representing from 58% (sheep) to 83% (rabbit) of the total lipid. The surfactant preparations were rather similar in lipid composition except that sheep surfactant contained about 10% lysophosphatidylcholine. 5. Hexadecanoic acid was the principal fatty acid. It was particularly high in phosphatidylcholine. 6. Phosphatidylglycerol was a minor constituent of all surfactants but phosphatidyldimethylethanolamine was not detected.
摘要
- 对来自牛、兔、大鼠和羊的肺表面活性剂进行了分离和分析。2. 所有制剂都含有高含量的花生四烯酰磷脂酰胆碱，并且能产生0.01 Nm-1或更低的稳定表面张力。3. 蛋白质含量为干重的8 - 18%。观察到多种蛋白质；它们的总体组成中疏水氨基酸残基含量较高。4. 脂质含量从79%（牛）到90%（兔）不等，磷脂酰胆碱占总脂质的58%（羊）到83%（兔）。除了羊表面活性剂含有约10%的溶血磷脂酰胆碱外，表面活性剂制剂在脂质组成上相当相似。5. 十六烷酸是主要脂肪酸。它在磷脂酰胆碱中含量特别高。6. 磷脂酰甘油是所有表面活性剂的次要成分，但未检测到磷脂酰二甲基乙醇胺。
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