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使用三柱串联放大的高速逆流色谱法从显齿蛇葡萄叶提取物中纯化（+）-二氢杨梅素

Purification of (+)-dihydromyricetin from leaves extract of Ampelopsis grossedentata using high-speed countercurrent chromatograph with scale-up triple columns.

作者信息

Du Qizhen, Cai Weijian, Xia Ming, Ito Yoichiro

机构信息

Institute of Food and Biological Engineering, Hangzhou University of Commerce, China.

出版信息

J Chromatogr A. 2002 Oct 11;973(1-2):217-20. doi: 10.1016/s0021-9673(02)01092-0.

DOI:10.1016/s0021-9673(02)01092-0
PMID:12437181
Abstract

Purification of (+)-dihydromyricetin from an extract (16 g) of leaves of Ampelopsis grossedentata was performed using a preparative triple-column countercurrent chromatograph. With a solvent system composed of n-hexane-ethyl acetate-methanol-water (1:3:2:4, v/v) 11.3 g of (+)-dihydromyricetin was obtained at a high purity of over 99% by HPLC at 254 nm in 9 h.

摘要

采用制备型三柱逆流色谱法从显齿蛇葡萄叶提取物（16克）中纯化（+）-二氢杨梅素。以正己烷-乙酸乙酯-甲醇-水（1:3:2:4，v/v）组成的溶剂系统，在9小时内通过254nm高效液相色谱法获得了11.3克高纯度（超过99%）的（+）-二氢杨梅素。

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