Du Qizhen, Li Lei, Jerz Gerold
Institute of Food and Biological Engineering, Zhemjiang Gongshang University, 149 Jiaogong Road, Hangzhou, Zhejiang 310035, China.
J Chromatogr A. 2005 Jun 3;1077(1):98-101. doi: 10.1016/j.chroma.2005.04.072.
Purification of astilbin from the rhizome extract of Smilax glabra was conducted using a high-speed counter-current chromatograph equipped with a 700 mL column. In a single operation, 1.5 g of crude sample was separated to yield 105 mg of component astilbin and 48 mg of isoastilbin while the upper phase of the two-phase solvent system composed of n-hexane-n-butanol-water (1:1:2, v/v/v) was used for stationary phase. The chemical structures of the two flavonoid glycosides were confirmed by electrospray ionization ion trap multiple mass spectrometry and nuclear magnetic resonance experiments.
使用配备700 mL柱的高速逆流色谱仪从光叶菝葜根茎提取物中纯化落新妇苷。单次操作中，将1.5 g粗样品进行分离，得到105 mg落新妇苷组分和48 mg异落新妇苷，同时使用正己烷-正丁醇-水（1:1:2，v/v/v）组成的两相溶剂系统的上相作为固定相。通过电喷雾电离离子阱多级质谱和核磁共振实验确定了这两种黄酮苷的化学结构。
