Huaihua Key Laboratory for Preparation of Ceramics Materials and Devices, Hunan Engineering Laboratory for Preparation Technology of Polyvinyl Alcohol Fiber Material, Huaihua University, Huaihua, PR China.
Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Guangxi University for Nationalities, Nanning, PR China.
PLoS One. 2020 Aug 17;15(8):e0237583. doi: 10.1371/journal.pone.0237583. eCollection 2020.
Identification and quantification of plant flavonoids are critical to pharmacokinetic study and pharmaceutical quality control due to their distinct pharmacological functions. Here we report on a novel plant flavonoid electrochemical sensor for sensitive and selective detection of dihydromyricetin (DMY) based on double- layered membranes consisting of gold nanoparticles (Au) anchored on reduced graphene oxide (rGO) and molecularly imprinted polymers (MIPs) modified glassy carbon electrode (GCE). Both rGO-Au and MIPs membranes were directly formed on GCE via in-situ electrochemical reduction and polymerization processes step by step. The compositions, morphologies, and electrochemical properties of membranes were investigated with X-ray powder diffractometry (XRD), Fourier transform infrared spectrum (FTIR), Field emission scanning electron microscopy (FESEM) combined with various electrochemical methods. The fabricated electrochemical sensor labeled as GCE│rGO-Au/MIPs exhibited excellent performance in determining of DMY under optimal experimental conditions. A wide linear detection range (LDR) ranges from 2.0×10-8 to 1.0×10-4 M together with a low limit of detection (LOD) of 1.2×10-8 M (S/N = 3) were achieved. Moreover, the electrochemical sensor was employed to determine DMY in real samples with satisfactory results.
由于植物类黄酮具有独特的药理功能，因此对其进行鉴定和定量分析对于药代动力学研究和药物质量控制至关重要。在此，我们报告了一种基于双层膜的新型植物类黄酮电化学传感器，用于灵敏和选择性检测二氢杨梅素（DMY），双层膜由锚定在还原氧化石墨烯（rGO）和分子印迹聚合物（MIPs）上的金纳米粒子（Au）组成，修饰玻碳电极（GCE）。rGO-Au 和 MIPs 膜通过原位电化学还原和逐步聚合过程直接在 GCE 上形成。采用 X 射线粉末衍射（XRD）、傅里叶变换红外光谱（FTIR）、场发射扫描电子显微镜（FESEM）结合各种电化学方法对膜的组成、形貌和电化学性质进行了研究。所制备的电化学传感器标记为 GCE│rGO-Au/MIPs，在最佳实验条件下对 DMY 的测定表现出优异的性能。实现了宽线性检测范围（LDR）从 2.0×10-8 到 1.0×10-4 M，检测限（LOD）低至 1.2×10-8 M（S/N = 3）。此外，该电化学传感器还用于测定实际样品中的 DMY，结果令人满意。
