Conti G, Portincasa P
Department of Pathology, Medical School, University of Parma, Italy.
New Microbiol. 2002 Oct;25(4):385-98.
The multiplication of Ulster 73 virus, an avian strain of type A influenza virus, was blocked in chick embryo fibroblast cells, CEF, by treatment with 0.5 microg/ml of chromomycin A3 whereas in LLC-MK2 cells no inhibition of replication was observed. Virus-induced polypeptide synthesis in chick embryo fibroblast cells was confined to the synthesis of PB2, PB1 and PA subunits of the RNA dependent-RNA polymerase, the nucleoprotein NP, the non-structural protein NS1, the haemagglutinin HA, the non-structural protein NS2; only the membrane M1 polypeptide synthesis was greatly inhibited. Viral unpolyadenylated cRNAs synthesis was studied at a late time of the infection, 8 hours p.i.: chromomycin A3 was able to inhibit the "novo" synthesis of complementary RNA poly(A)- and segment 7 of virion RNA. The mode of action of the drug in chick embryo fibroblast cells is discussed.
用0.5微克/毫升的放线菌素A3处理鸡胚成纤维细胞（CEF），可阻断甲型流感病毒的一种禽类毒株阿尔斯特73病毒的增殖，而在LLC-MK2细胞中未观察到对病毒复制的抑制作用。病毒诱导的鸡胚成纤维细胞中的多肽合成局限于依赖RNA的RNA聚合酶的PB2、PB1和PA亚基、核蛋白NP、非结构蛋白NS1、血凝素HA、非结构蛋白NS2的合成；只有膜M1多肽的合成受到极大抑制。在感染后期（感染后8小时）研究了病毒非聚腺苷酸化cRNAs的合成：放线菌素A3能够抑制互补RNA聚（A）-和病毒粒子RNA第7节段的“从头”合成。讨论了该药物在鸡胚成纤维细胞中的作用方式。
