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胸膜肺炎放线杆菌2型原型活负标记疫苗的构建

Construction of an Actinobacillus pleuropneumoniae serotype 2 prototype live negative-marker vaccine.

作者信息

Tonpitak Walaiporn, Baltes Nina, Hennig-Pauka Isabel, Gerlach Gerald F

机构信息

Institut für Mikrobiologie und Tierseuchen, Tieraerztliche Hochschule Hannover, Germany.

出版信息

Infect Immun. 2002 Dec;70(12):7120-5. doi: 10.1128/IAI.70.12.7120-7125.2002.

DOI:10.1128/IAI.70.12.7120-7125.2002
PMID:12438394
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC133062/
Abstract

Deletions were introduced into the ureC and apxIIA genes of an Actinobacillus pleuropneumoniae serotype 2 strain by homologous recombination and counterselection. The double-mutant contains no foreign DNA, is highly attenuated, protects pigs from homologous challenge upon a single aerosol application, and facilitates the serological discrimination of immunized and infected herds.

摘要

通过同源重组和反选择，将缺失引入胸膜肺炎放线杆菌2型菌株的ureC和apxIIA基因中。该双突变体不含外源DNA，高度减毒，单次气溶胶接种即可保护猪免受同源攻击，并有助于对免疫和感染猪群进行血清学鉴别。

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Construction of an Actinobacillus pleuropneumoniae serotype 2 prototype live negative-marker vaccine.胸膜肺炎放线杆菌2型原型活负标记疫苗的构建
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本文引用的文献

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Selected topics from classical bacterial genetics.经典细菌遗传学选讲
Curr Protoc Mol Biol. 2002 Aug;Chapter 1:Unit 1.4. doi: 10.1002/0471142727.mb0104s59.
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Both transferrin binding proteins are virulence factors in Actinobacillus pleuropneumoniae serotype 7 infection.两种转铁蛋白结合蛋白都是胸膜肺炎放线杆菌7型感染中的毒力因子。
FEMS Microbiol Lett. 2002 Apr 9;209(2):283-7. doi: 10.1111/j.1574-6968.2002.tb11145.x.
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Actinobacillus pleuropneumoniae iron transport and urease activity: effects on bacterial virulence and host immune response.胸膜肺炎放线杆菌的铁转运与脲酶活性：对细菌毒力和宿主免疫反应的影响
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A novel strategy for protective Actinobacillus pleuropneumoniae subunit vaccines: detergent extraction of cultures induced by iron restriction.一种用于保护性胸膜肺炎放线杆菌亚单位疫苗的新策略：通过铁限制诱导培养物的去污剂提取
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Intramuscular immunization with genetically inactivated (ghosts) Actinobacillus pleuropneumoniae serotype 9 protects pigs against homologous aerosol challenge and prevents carrier state.用基因灭活（空壳菌）的胸膜肺炎放线杆菌9型进行肌肉注射免疫可保护猪免受同源气溶胶攻击并防止携带状态。
Vaccine. 2000 Jul 1;18(26):2945-55. doi: 10.1016/s0264-410x(00)00107-9.
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Evaluation of an indirect enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to the Apx toxins of Actinobacillus pleuropneumoniae.用于检测胸膜肺炎放线杆菌Apx毒素抗体的间接酶联免疫吸附测定（ELISA）的评估
Vet Microbiol. 2000 Jan;71(1-2):81-7. doi: 10.1016/s0378-1135(99)00157-1.
10
Pigs aerogenously immunized with genetically inactivated (ghosts) or irradiated Actinobacillus pleuropneumoniae are protected against a homologous aerosol challenge despite differing in pulmonary cellular and antibody responses.用基因灭活（菌影）或经辐射的胸膜肺炎放线杆菌经气溶胶免疫的猪，尽管肺部细胞和抗体反应不同，但仍能抵御同源气溶胶攻击。
J Biotechnol. 1999 Aug 20;73(2-3):251-60. doi: 10.1016/s0168-1656(99)00143-1.