钩端螺旋体在体外可通过需氧反应和非需氧反应被杀死。
Leptospires are killed in vitro by both oxygen-dependent and -independent reactions.
作者信息
Murgia Rossella, Garcia Rodolfo, Cinco Marina
机构信息
Department of Biomedical Sciences, University of Trieste, Italy.
出版信息
Infect Immun. 2002 Dec;70(12):7172-5. doi: 10.1128/IAI.70.12.7172-7175.2002.
Abstract
This study reports for the first time that leptospires are killed by H(2)O(2) and by low-molecular-weight primary granule components, which are agents normally released by neutrophils upon stimulation. Although both pathogenic and nonpathogenic strains were sensitive to H(2)O(2)-mediated killing, nonpathogenic organisms were found to be more susceptible. In addition, the killing of leptospires by H(2)O(2) was found to be independent of the presence of the neutrophil primary granule component myeloperoxidase and therefore not a consequence of halogenation reactions. We have also determined that leptospires are significantly sensitive only to primary granule components and, among those, to proteins and/or peptides of less than 30 kDa.
摘要
本研究首次报告，钩端螺旋体可被过氧化氢（H₂O₂）以及低分子量初级颗粒成分杀死，这些成分是中性粒细胞受刺激后通常释放的物质。尽管致病性和非致病性菌株对H₂O₂介导的杀伤均敏感，但发现非致病性生物体更易受影响。此外，还发现H₂O₂对钩端螺旋体的杀伤与中性粒细胞初级颗粒成分髓过氧化物酶的存在无关，因此不是卤化反应的结果。我们还确定，钩端螺旋体仅对初级颗粒成分敏感，且在这些成分中，对分子量小于30 kDa的蛋白质和/或肽敏感。
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