Wang B, Sullivan J A, Sullivan G W, Mandell G L
Infect Immun. 1984 Dec;46(3):809-13. doi: 10.1128/iai.46.3.809-813.1984.
It has previously been shown that human neutrophils ingest and kill nonpathogenic Leptospira biflexa in the absence of serum but that pathogenic Leptospira interrogans is not ingested by neutrophils even in the presence of normal serum. We extended this study by examining the interactions of human monocytes and monocyte-derived macrophages with pathogenic L. interrogans (serovar icterohaemorrhagiae) and evaluating the opsonizing effect of serotype-specific immune serum on the phagocytosis of pathogenic leptospires by monocytes and neutrophils. Leptospires were incubated with monocytes in pellets at 37 degrees C in 5% CO2. No ingestion or killing of pathogenic leptospires occurred when 10% normal serum was used. However, when the pathogenic leptospires were pretreated with serotype-specific immune serum, monocytes or neutrophils in pellets ingested 96% of the organisms and killed 94% of those ingested. Microscopic observations of the interaction confirmed that phagocytosis of the opsonized pathogenic leptospires by monocytes, monocyte-derived macrophages, and neutrophils had occurred. The opsonizing effect of specific antibody may play an important role in the mechanism of host defense against leptospirosis.
此前已有研究表明，人类中性粒细胞在无血清的情况下可摄取并杀死非致病性双曲钩端螺旋体，但即使在存在正常血清的情况下，致病性问号钩端螺旋体也不会被中性粒细胞摄取。我们通过研究人类单核细胞和单核细胞衍生的巨噬细胞与致病性问号钩端螺旋体（黄疸出血血清型）的相互作用，并评估血清型特异性免疫血清对单核细胞和中性粒细胞吞噬致病性钩端螺旋体的调理作用，扩展了这项研究。将钩端螺旋体与单核细胞在含有5%二氧化碳的37摄氏度环境下的沉淀中共同孵育。当使用10%的正常血清时，未发生对致病性钩端螺旋体的摄取或杀伤。然而，当致病性钩端螺旋体用血清型特异性免疫血清预处理后，沉淀中的单核细胞或中性粒细胞摄取了96%的菌体，并杀死了94%摄取的菌体。对相互作用的显微镜观察证实，单核细胞、单核细胞衍生的巨噬细胞和中性粒细胞对调理后的致病性钩端螺旋体发生了吞噬作用。特异性抗体的调理作用可能在宿主防御钩端螺旋体病的机制中起重要作用。
