Adam Gregory C, Sorensen Erik J, Cravatt Benjamin F
The Skaggs Institute for Chemical Biology and the Department of Chemistry, The Scripps Research Institute, La Jolla, California 92037, USA.
Mol Cell Proteomics. 2002 Oct;1(10):781-90. doi: 10.1074/mcp.r200006-mcp200.
With complete genome sequences now available for several prokaryotic and eukaryotic organisms, biological researchers are charged with the task of assigning molecular and cellular functions to thousands of predicted gene products. To address this problem, the field of proteomics seeks to develop and apply methods for the global analysis of protein expression and protein function. Here we review a promising new class of proteomic strategies that utilizes synthetic chemistry to create tools and assays for the characterization of protein samples of high complexity. These approaches include the development of chemical affinity tags to measure the relative expression level and post-translational modification state of proteins in cell and tissue proteomes. Additionally, we discuss the emerging field of activity-based protein profiling, which aims to synthesize and apply small molecule probes that monitor dynamics in protein function in complex proteomes.
随着几种原核生物和真核生物的完整基因组序列现已可用，生物学研究人员肩负着为数千种预测的基因产物赋予分子和细胞功能的任务。为了解决这个问题，蛋白质组学领域致力于开发和应用用于蛋白质表达和蛋白质功能全局分析的方法。在这里，我们综述了一类有前景的新型蛋白质组学策略，这些策略利用合成化学来创建工具和检测方法，以表征高度复杂的蛋白质样品。这些方法包括开发化学亲和标签，以测量细胞和组织蛋白质组中蛋白质的相对表达水平和翻译后修饰状态。此外，我们还讨论了基于活性的蛋白质谱分析这一新兴领域，该领域旨在合成和应用小分子探针，以监测复杂蛋白质组中蛋白质功能的动态变化。
