胞磷胆碱对小鼠培养视网膜中受损的视网膜神经节细胞具有保护作用。
Citicoline has a protective effect on damaged retinal ganglion cells in mouse culture retina.
作者信息
Oshitari Toshiyuki, Fujimoto Naoya, Adachi-Usami Emiko
机构信息
Department of Ophthalmology and Visual Science, Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba 260-8670, Japan.
出版信息
Neuroreport. 2002 Nov 15;13(16):2109-11. doi: 10.1097/00001756-200211150-00023.
Some clinical reports indicate that exogenous CDP-choline (citicoline) may have a therapeutic effect in patients with glaucoma. However, the precise effect of citicoline on damaged retinal ganglion cells (RGCs) remains to be explained. We performed tissue culture of mouse retinal explants and investigated the effect of citicoline on damaged RGCs by the quantitative analysis of TdT-dUTP terminal nick-end labeling (TUNEL) staining and the assessment of the number of regenerating neurites. The TUNEL-positive ratio in 0.1-10 micromol/l citicoline-treated retina was very low, and the number of regenerating neurites increased more than in control retina. Our findings suggest that citicoline has a protective effect on damaged RGCs in tissue culture of retina.
一些临床报告表明，外源性胞二磷胆碱（西地胆碱）可能对青光眼患者具有治疗作用。然而，西地胆碱对受损视网膜神经节细胞（RGCs）的确切作用仍有待阐明。我们进行了小鼠视网膜外植体的组织培养，并通过TdT-dUTP末端脱氧核苷酸转移酶介导的缺口末端标记（TUNEL）染色的定量分析和对再生神经突数量的评估，研究了西地胆碱对受损RGCs的影响。在0.1 - 10微摩尔/升西地胆碱处理的视网膜中，TUNEL阳性率非常低，并且再生神经突的数量比对照视网膜增加得更多。我们的研究结果表明，在视网膜组织培养中，西地胆碱对受损的RGCs具有保护作用。
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