Ramli U S, Baker D S, Quant P A, Harwood J L
Malaysian Palm Oil Board, P.O. Box 10620, 50700 Kuala Lumpur, Malaysia.
Biochem Soc Trans. 2002 Nov;30(Pt 6):1043-6. doi: 10.1042/bst0301043.
Control analysis is a powerful method to quantify the regulation of metabolic pathways. We have applied it to lipid biosynthesis for the first time by using model tissue culture systems from the important oil crops, olive ( Olea europaea L.) and oil palm ( Elaeis guineensis Jacq.). By the use of top-down control analysis, fatty acid biosynthesis has been shown to exert more control than lipid assembly under different experimental conditions. However, both parts of the lipid biosynthetic pathway are important, so that attempts to alter oil yield by manipulating the activity of a single enzyme step are very unlikely to produce significant increases.
控制分析是一种用于量化代谢途径调控的强大方法。我们首次通过使用来自重要油料作物橄榄（油橄榄）和油棕的模型组织培养系统，将其应用于脂质生物合成。通过自上而下的控制分析，已表明在不同实验条件下，脂肪酸生物合成比脂质组装发挥更大的控制作用。然而，脂质生物合成途径的两个部分都很重要，因此试图通过操纵单个酶步骤的活性来提高油产量极不可能产生显著增加。
