Zvara Peter, Kliment Jan, DeRoss Anthony L, Irwin Brian H, Malley Susan E, Plante Mark K, Vizzard Margaret A
Department of Surgery, University of Vermont College of Medicine, Burlington, USA.
J Urol. 2002 Dec;168(6):2682-8. doi: 10.1016/S0022-5347(05)64244-9.
We validated a male rat model of bladder outflow obstruction and compared the expression of bladder neurotrophic factor mRNA in male and female rats 6 weeks after bladder outlet obstruction.
We examined the proximal urethra in male Wistar rats. Urethral lumen reducing ligatures were placed in 15 females and 19 males, while 10 male and 10 female controls underwent sham surgery. Awake cystometry was performed 6 weeks after surgery. Ribonuclease protection assay was used to measure changes in bladder neurotrophic factor mRNA expression in the 2 sexes.
Average bladder capacity in rats with bladder outlet obstruction increased 3-fold in males and 4.4-fold in females compared with controls, while bladder weight increased 2.2 and 4.3-fold, respectively. Filling and threshold pressure increased significantly and nonvoiding bladder contractions were recorded in 100% of female and 80% of male rats with bladder outlet obstruction. An 8-fold increase in bladder brain derived neurotrophic factor mRNA was noted in each sex after obstruction. A 2-fold increase in bladder nerve growth factor mRNA after obstruction was only observed in females.
This male rat model of bladder outlet obstruction was created by placing lumen reducing ligatures at the urethrovesical junction. The dramatic increase in bladder brain derived neurotrophic factor mRNA expression and differential expression of nerve growth factor mRNA in male and female rats with bladder outlet obstruction suggest that additional neurotrophic factors may contribute to the lower urinary tract neuroplasticity associated with bladder outlet obstruction and this contribution may be gender dependent.
我们验证了一种雄性大鼠膀胱出口梗阻模型，并比较了膀胱出口梗阻6周后雄性和雌性大鼠膀胱神经营养因子mRNA的表达。
我们检查了雄性Wistar大鼠的近端尿道。对15只雌性和19只雄性大鼠进行尿道腔缩小结扎，而10只雄性和10只雌性对照大鼠接受假手术。术后6周进行清醒膀胱测压。采用核糖核酸酶保护试验来测量两性膀胱神经营养因子mRNA表达的变化。
与对照组相比，膀胱出口梗阻大鼠的平均膀胱容量在雄性中增加了3倍，在雌性中增加了4.4倍，而膀胱重量分别增加了2.2倍和4.3倍。膀胱出口梗阻的雌性大鼠100%和雄性大鼠80%记录到充盈压和阈值压力显著增加且出现无排尿膀胱收缩。梗阻后两性膀胱脑源性神经营养因子mRNA均增加8倍。梗阻后仅在雌性大鼠中观察到膀胱神经生长因子mRNA增加2倍。
这种雄性大鼠膀胱出口梗阻模型是通过在尿道膀胱交界处放置缩小管腔的结扎线建立的。膀胱出口梗阻的雄性和雌性大鼠中膀胱脑源性神经营养因子mRNA表达的显著增加以及神经生长因子mRNA的差异表达表明，其他神经营养因子可能促成了与膀胱出口梗阻相关的下尿路神经可塑性，且这种作用可能存在性别依赖性。
