Podraza Wojciech, Gonet Bolesław, Kordek Agnieszka
Department of Medical Physics, Pomeranian Academy of Medicine, Szczecin, Poland.
Luminescence. 2002 Nov-Dec;17(6):381-5. doi: 10.1002/bio.700.
The ultra-weak light, chemiluminescence (CL), of stimulated leukocytes is a well-known phenomenon. Parameters of this CL are modified by many factors including laboratory procedures. The order of stimulation and enhancement (two possibilities) and two concentrations of luminol create four types of procedure, which were accomplished in five sample storage 'time points'. We received the strongest signals of CL using higher concentrations of luminol (and DMSO), but only when stimulation (FMLP) was used before enhancement (luminol); luminol used before FMLP strongly inhibited CL. For lower luminol concentration (and DMSO), the order of stimulation and enhancement was of no importance. There were comparable but weaker signals of CL in this case. We received stronger signals with storage time for all procedures. It may be dependent on the priming of phagocytes by releasing cell factors. Stimulation (FMLP) before enhancement (luminol) eliminates the inhibitory effect of DMSO on CL.
受刺激白细胞发出的超微弱光即化学发光（CL）是一种众所周知的现象。这种化学发光的参数会受到包括实验室操作在内的许多因素的影响。刺激和增强的顺序（两种可能性）以及鲁米诺的两种浓度产生了四种操作类型，这些操作在五个样本储存“时间点”完成。我们使用较高浓度的鲁米诺（和二甲基亚砜）时获得了最强的化学发光信号，但前提是刺激（甲酰甲硫氨酸-亮氨酸-苯丙氨酸，FMLP）在增强（鲁米诺）之前进行；在FMLP之前使用鲁米诺会强烈抑制化学发光。对于较低浓度的鲁米诺（和二甲基亚砜），刺激和增强的顺序并不重要。在这种情况下，化学发光信号较弱但具有可比性。所有操作中，随着储存时间的延长，我们获得了更强的信号。这可能取决于通过释放细胞因子对吞噬细胞的启动作用。增强（鲁米诺）之前进行刺激（FMLP）可消除二甲基亚砜对化学发光的抑制作用。
