Ristola M, Repo H
Department of Bacteriology and Immunology, University of Helsinki, Finland.
J Biolumin Chemilumin. 1990 Jul-Sep;5(3):155-60. doi: 10.1002/bio.1170050302.
Whole blood luminol-enhanced chemiluminescence (CL) was studied using N-formyl-methionyl-leucyl-phenylalanine (FMLP) or opsonized zymosan particles as stimuli. The peak and the integral responses of CL were recorded. In the FMLP-induced CL the initial activation (1-minute values) was also studied, because it coincides with the extracellular production of oxy radicals. Correction factors based on neutrophil count and on haemoglobin concentration were found to decrease dispersion and shape the distributions of the CL responses close to normal in a study of 50 healthy adults. One-minute values were significantly lower in women than in men but there were no significant differences for peak or integral values between sexes. Depressed reaction is in accordance with the previous findings that phagocytic oxy radical production is depressed in female plasma. Thus, our results suggest that 1-minute value is a variable more sensitive than peak or integral value of the CL response.
使用N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）或调理酵母聚糖颗粒作为刺激物，研究了全血鲁米诺增强化学发光（CL）。记录了CL的峰值和积分响应。在FMLP诱导的CL中，还研究了初始激活（1分钟值），因为它与细胞外氧自由基的产生相吻合。在一项对50名健康成年人的研究中，发现基于中性粒细胞计数和血红蛋白浓度的校正因子可减少离散度，并使CL反应的分布形状接近正常。女性的1分钟值明显低于男性，但两性之间的峰值或积分值没有显著差异。反应降低与先前的研究结果一致，即女性血浆中吞噬性氧自由基的产生受到抑制。因此，我们的结果表明，1分钟值是比CL反应的峰值或积分值更敏感的变量。
